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Dasatinib group buy from Nyles

dasatinib senolytic senescent scenescent cells sasp senolytics group buy

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#31 Logic

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Posted 31 July 2016 - 03:40 PM

OK,

I'm working in Iraq and I have crappy bandwidth and work 72+ hour weeks. So I have not had time to catch up with the thread, and if all I need is 300 mg every 3 - 6 months, then obviously I don't need so much.

 

So put me in form 6 grams, I'd like to try this for awhile.

 

My assumption was that you took it everyday when you take your quercetin. Apparently not...

 

 

This buy has nothing to do with Nyles, SearchingForAnswers.  

I just used this thread as my time is spent updating group buy spreadsheets and answering PMs nowadays.

 

It will come from the same supplier as the Nilotinib and be tested by the same lab.
http://www.longecity...oup-buy/page-15

 

From 40 to 6 grams;  damn!  I shouldn't have said anything!  :laugh:



#32 Logic

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Posted 31 July 2016 - 04:03 PM

5 grams would be no problem. Is it powder or caps?

Assuming this is done with QC, will you provide the report on an external webpage for us to confirm or do we have to take your word for it?

 

Its powder Cosmicalstorm.

 

So far I am leaning toward tested D at $ 5.82 per gram, rather than untested at $ 4.62 per gram?

The supplier has proven reliable in the 2 Nilotinib buys, but I think the piece of mind etc provided by having it tested is worth the extra $ 1.20 per gram?

What does everyone else think?

 

The testing report will be provided here, but redacted as requested by the lab.

As with the Nilotinib, I will provide the unredacted report to long standing, trusted members of the buy that request it, on the understanding that they honour the lab's requested confidentiality.

This verifies its authenticity for everyone else.



#33 Logic

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Posted 31 July 2016 - 04:14 PM

Dasitinib Group Buy.  By Logic

 

SearchingForAnswers    6 grams
Niner                               10
Logic                               10
Fafner55                         10

mikey                                5

Cosmicalstorm                 5

Total                                46

 

454 grams to go to get to the 500 gram minimum.

 

Please state your preference regard testing or not.  

ie:  $ 5.82/g tested, or $ 4.62 untested.

 

Please 'advertise' the buy so we can get to the 500 gram minimum!



#34 Fafner55

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Posted 31 July 2016 - 07:53 PM

Regarding dosage, starting from “The Achilles’ Heel of Senescent Cells: From Transcriptome to Senolytic Drugs” (2015) http://onlinelibrary.wiley.com/doi/10.1111/acel.12344/full

A single dose of D+Q (D: 5 mg kg−1 body weight and Q: 50 mg kg−1 by oral gavage here and in the following studies), a drug ratio that was most effective in senescent MEFs (data not shown), reduced SA-βgal+ cells (Fig. 3C) and p16 mRNA (Fig. 3D) in fat from old mice within 5 days. D+Q also reduced p16-positive cells in liver from old mice (Fig. 3E-F). As with AP20187, the drug that activates selective killing of cells expressing p16 in transgenic INK-ATTAC mice (Baker et al., 2011), not all senescent cells were removed by D+Q.

 

A direct scaling of this 5 mg/kg body weight dose of dasatinib to my weight gives (5 mg/kg)(70 kg) = 350 mg.  

This dose is twice the “escalation dose” recommended for cancer treatment in humans and is unsafe.

 

The Human Equivalent Dose (HED) scaling from mice to 70 kg adult humans is

http://www.fasebj.org/content/22/3/659.full.pdf+html  

(5 mg/kg)(3/37)(70 kg) = 28.4 mg

 

I did not see much if any benefit at 70 mg Dasatinib + 6400 mg Quercetin dihydrate.  

 

Increasing the dose to 120 mg Dasatinib + 6400 mg quercetin and adding 2000 mg honokiol cleared some of the sun/age damage to my skin.

 

For further confirmation, from the plasma concentration-time profile shown in Figure 1 of “Metabolism and Disposition of Dasatinib after Oral Administration to Humans” (2008) http://dmd.aspetjour...t/36/7/1357.fula 100 mg dose of Dasatinib results in a peak serum concentration of 104.5 ng/ml after 0.5 hr with a half-life of 3.6 hours.  Converting to moles/liter, this peak concentration is calculated to be  

= (104.5 ng/ml)/(488.01 ng/nMol)

= 0.214 nM/ml

= 214 nM/l, which is close to a reasonable target of 200 nM/L taken from Figure 2A of “The Achilles’ Heel”

 

 

Here is what I currently take: on an empty stomach I ingest

  1. 120 mg Dasatinib
  2. 2000 mg Quercetin phytosome
  3. 2000 mg Honokiol (Magnolia bark extract)
  4. 500 mg Pterostilbene
  5. 600 mg Sodium butyrate every 15 minutes, 8 total, starting 15 minutes before taking anything else

 

I am certain that there is a strong apoptotic effect from this combination since my skin has gotten progressively smoother with repeated doses and some keratoses have either disappeared or been greatly reduced.

 

The major side effects I experience is fatigue and less mental clarity, which lasts maybe 5 days.  On two occasions there was diarrhea.

 

Dasatinib is a powerful drug that is not without side effects or interactions.

  1. “Phase I Dose-Escalation and Pharmacokinetic Study of Dasatinib in Patients with Advanced Solid Tumors” (2009)  http://clincancerres.aacrjournals.org/content/15/19/6232.short

  2. http://www.cancerresearchuk.org/about-cancer/cancers-in-general/treatment/cancer-drugs/dasatinib

  3. http://www.drugs.com/cdi/dasatinib.html

  4. http://en.wikipedia.org/wiki/Dasatinib

 

Side effects and contraindications include

  1. Do not take dasatinib if you are sick.  Dasatinib increases the risk of getting an infection from a drop in white blood cells – it is harder to fight infections and you can become very ill. You may have headaches, aching muscles, a cough, a sore throat, pain passing urine, or you may feel cold and shivery.

  2. 30% develop diarrhea

  3. 30% experience headaches

  4. 20% develop a rash or red, dry, itchy skin

  5. 20% experience fluid buildup in the body

  6. 20% experience a feeling of being sick

  7. 20% experience fatigue

  8. 10% feel bone and muscle pain

 


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#35 Logic

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Posted 01 August 2016 - 08:36 PM

Thx Fafner55.
As I recall, no-one has experimented with D&Q as much as you have, so your experience and input are much appreciated.


  • Agree x 2

#36 SearchingForAnswers

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Posted 07 August 2016 - 02:42 PM

 

OK,

I'm working in Iraq and I have crappy bandwidth and work 72+ hour weeks. So I have not had time to catch up with the thread, and if all I need is 300 mg every 3 - 6 months, then obviously I don't need so much.

 

So put me in form 6 grams, I'd like to try this for awhile.

 

My assumption was that you took it everyday when you take your quercetin. Apparently not...

 

 

This buy has nothing to do with Nyles, SearchingForAnswers.  

I just used this thread as my time is spent updating group buy spreadsheets and answering PMs nowadays.

 

It will come from the same supplier as the Nilotinib and be tested by the same lab.
http://www.longecity...oup-buy/page-15

 

From 40 to 6 grams;  damn!  I shouldn't have said anything!  :laugh:

 

 

No problem.

 



#37 rarefried

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Posted 07 August 2016 - 07:23 PM

I'm in for the grams (tested preferred).



#38 Logic

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Posted 21 August 2016 - 01:37 PM

I'm in for the grams (tested preferred).

 

Thx Rarefied.

How many grams?  10?



#39 Logic

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Posted 21 August 2016 - 02:11 PM

Dasitinib Group Buy.  By Logic

SearchingForAnswers    6 grams
Niner                               10
Logic                               10
Fafner55                         10
mikey                                5
Cosmicalstorm                  5

Rarefied                           ?

ClarkSims                         20

Pwain                               10?
Total                                66+

 

434 - grams to go to get to the 500 gram minimum.

I will see if I can get the minimum reduced, but that will probably push the price up.

 

Please state your preference regard testing or not. 
ie:  $ 5.82/g tested, or $ 4.62 untested.

 

If we do not reach 500 grams at around the same time as a Nilotinib buy; it will cost $ 80.00 for shipping to the lab.

Please 'advertise' the buy so we can get to the 500 gram minimum!


Edited by Logic, 21 August 2016 - 02:18 PM.


#40 rarefried

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Posted 21 August 2016 - 08:01 PM

Yes, 10 grams, thanks Logic.



#41 Nate-2004

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Posted 26 August 2016 - 01:51 PM

I'm in for 10 grams, what's the standard dosage?



#42 Logic

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Posted 09 September 2016 - 11:16 PM

I got some prices on lower amounts as we probably wont reach 500 grams of something that will be used so seldomly.

 

100g at $ 6.60 per gram

50g   at $ 9.10 per gram

 

 

A courier fee of $ 70.00 needs to be added to any parcel coming in and should actually be shared equally amongst the total # of grams of whatever is arriving.
ie: if 500 grams of Nilotinib arrive and 100 grams of Dasatinib, we have a total of 600 grams at a courier cost of $ 0.12 per gram.

I feel that 100 grams is probably doable, so:

 

100g at $ 6.72 per gram
50g   at $ 9.22 per gram


I hope everyone is happy to go for a target of 100 grams?

Please post your thoughts and I will update the list.

 

 

 

 

 



#43 Logic

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Posted 09 September 2016 - 11:31 PM

I'm in for 10 grams, what's the standard dosage?

 

Thx Nate

 

 

The dosage:

 

D is:  0.4054054054054054 mg/kg = 28.4mg/70kg human
Q is:  4.054054054054054 mg/kg = 283.8 mg/70kg human

 

http://www.longecity...ndpost&p=722108

http://www.longecity...ndpost&p=784422

 

 

CLA looks like an interersting compound to add:

http://www.longecity...point/?p=788831

 

There a a number of other interesting compounds and anecdotal reports, making the main thread well worth reading:

http://www.longecity...nds-healthspan/



#44 Nate-2004

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Posted 09 September 2016 - 11:43 PM

So I'm 78 to 80 Kg that means 2.5g a day for 5 days would that even do anything?

#45 Logic

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Posted 22 September 2016 - 10:02 AM

So I'm 78 to 80 Kg that means 2.5g a day for 5 days would that even do anything?

 

Yes it will!

It will probably kill all the cells in your body!   :laugh:

 

You need 0.405mg of Dasatinib per kg of body weight.

In your case 0.405 mg/kg X 80 kg = 32.4 mg of Dasatinib.

Q is:  4.054 mg/kg X 80...



#46 Nate-2004

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Posted 22 September 2016 - 12:58 PM

Ohhh I somehow read that wrong. I wasn't even thinking about quercetin. I've already taken quercetin for like 2 months at around 2-3g /day, pretty sure I've killed all the senescent endothelial cells it's going to kill. 10g should last me a whole year then if it's just 32mg a day.


Edited by Nate-2004, 22 September 2016 - 12:58 PM.


#47 Logic

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Posted 23 September 2016 - 04:41 AM

Ohhh I somehow read that wrong. I wasn't even thinking about quercetin. I've already taken quercetin for like 2 months at around 2-3g /day, pretty sure I've killed all the senescent endothelial cells it's going to kill. 10g should last me a whole year then if it's just 32mg a day.

 

 

Longer:

IIRC the studies used a once of dose to see the results they did in the rats, so its the kind of thing one would take once every six months or so according to the study.
Do read the main thread however to get a better idea of the dosage protocols and results seen by Fafner55 and others.

I don't recall why offhand, but Quercetin also struck me as something to be taken infrequently, due to an undesirable epigenetic effect IIRC.



#48 Nate-2004

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Posted 24 September 2016 - 04:17 AM

32mg every six months? That would last years, what's the shelf life on that 10g?


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#49 Logic

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Posted 02 October 2016 - 03:02 PM

Dasitinib Group Buy.  By Logic

 

SearchingForAnswers       6 grams
Niner                               10
Logic                               10
Fafner55                         10
mikey                                5
Cosmicalstorm                  5
Rarefied                           10
ClarkSims                         20

Nate-2004                        10

Total                                80

Fafner55 has very kindly offered to: "make up the financial difference between a minimum order and the commitments you have received"

Thankyou Fafner55!
I will PM you as soon as I have posted here.
It would seem that everyone is pretty set on the 500 gram price....  :)



#50 ceridwen

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Posted 02 October 2016 - 10:07 PM

Could you count me in for 5 or is it too late?

#51 tunt01

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Posted 03 October 2016 - 01:07 AM

I'll take 5 g of Dasitinib



#52 Logic

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Posted 03 October 2016 - 10:13 AM

Could you count me in for 5 or is it too late?


Its not too late
Keep em coming people; the more the merrier! :)

Total: 90 grams


Edited by Logic, 03 October 2016 - 10:18 AM.


#53 Authentic

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Posted 03 October 2016 - 10:38 PM

Hi I'll take 2g of Dasatinib.

 


Edited by Authentic, 03 October 2016 - 10:54 PM.


#54 Authentic

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Posted 03 October 2016 - 10:41 PM

Sorry, make that 5g !!

 

I'm in for 5 :)



#55 ceridwen

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Posted 04 October 2016 - 01:44 AM

In for 2g
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#56 ceridwen

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Posted 08 October 2016 - 12:27 PM

Count me out again. Sorry
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#57 mkp6019

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Posted 17 October 2016 - 08:20 PM

I would be interested in participating in this group buy of 5 grams of dasatinib.



#58 DareDevil

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Posted 23 October 2016 - 09:40 AM

I'm definitely in for 10 grams.

 

I have already tried prescription Dasatinib taking 20mg/day with Quercitin for several weeks.

 

Its effect was noticeable enough to warrant me seeking higher dosage but it was prohibitively expensive.

 

This group buy is a Godsend. Thanks !

 

DareDevil

http://www.longecity...10-to-20-years/



#59 SearchingForAnswers

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Posted 24 October 2016 - 11:49 AM

Could you count me in for 5 or is it too late?


Its not too late
Keep em coming people; the more the merrier! :)

Total: 90 grams

Could you count me in for 5 or is it too late?


Its not too late
Keep em coming people; the more the merrier! :)

Total: 90 grams

Please Up my order from 6 to 20 grams tested.

#60 Captain Obvious

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Posted 25 October 2016 - 07:20 AM

Hi, just found this topic.

Please, count me in for 5 grams if international airmailing is not an issue.

I could go for 10 g, but I'm unsure of the dosage (20-30 mg per day vs every couple of months) and how long it would keep in a freezer.

 


Edited by Captain Obvious, 25 October 2016 - 07:50 AM.






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