680 replies to this topic

[C0rt3x]

I have ordered the 100g of HEPPS, hopefully will be delivered in a few days.

 

 

[C0rt3x]

They have cancelled my order

[Turnbuckle]

Could be a nice little business -- buying it from China, repackaging it and selling it on ebay at a 500% markup.

[kurt9]

I'm wondering if the appropriate solution is to set up an LLC, complete with DBA and like, before attempting to buy anything from a research lab. First, these guys are concerned about liability if someone buys their products and is injured as a result. Second, they are used to selling to commercial accounts such as other research labs. They may want to be sure you are a commercial account before they sell to you.

[C0rt3x]

I'm wondering if the appropriate solution is to set up an LLC, complete with DBA and like, before attempting to buy anything from a research lab. First, these guys are concerned about liability if someone buys their products and is injured as a result. Second, they are used to selling to commercial accounts such as other research labs. They may want to be sure you are a commercial account before they sell to you.

 

I emailed them to explain that I wan an individual and not a business, they accepted that and told me to go ahead and order, so I did but then they cancelled.

I am waiting for their reply.

[Turnbuckle]

You are dealing with clerks at a big corporation. They have rules they can't break, so if you don't check the right boxes, you won't get what you want. 

[bladedmind]

An easier way to get HEPPS is at Amazon, from legit vendor RPI, 100 mg (or one kg).

 

https://www.amazon.com/dp/B01N5LTPCF

 

The RPI website offers 25 mg.  

 

 

[EliotH]

An easier way to get HEPPS is at Amazon, from legit vendor RPI, 100 mg (or one kg).

 

https://www.amazon.com/dp/B01N5LTPCF

 

The RPI website offers 25 mg.  

 

I posted the link on the previous page but apparently no one noticed. I ordered and received it already, even though Amazon said expected delivery would next month. It actually came in a box from netascientific. They offer more options but I don't know if they ship to individuals - haven't had the need to try. They all have the same CAS number so I am pretty sure it is the right stuff.

 

https://www.netascientific.com/ - just enter HEPPS in the search box.

[Phoebus]

Apologies if this has already been posted 

 

 

 

Oleuropein-Rich Olive Leaf Extract Attenuates Neuroinflammation in the Alzheimer’s Disease Mouse Model

•  

Cite this: ACS Chem. Neurosci. 2022, 13, 7, 1002–1013

Publication Date:March 9, 2022

 

https://doi.org/10.1...emneuro.2c00005

 

 

ACS Chemical Neuroscience

Abstract

Alzheimer’s disease (AD) is the most common form of dementia among several neurodegenerative disorders afflicting the elderly. AD is characterized by the deposition of extracellular amyloid-β (Aβ) plaques, disrupted blood–brain barrier (BBB), and neuroinflammation. Several studies have demonstrated the health benefits of olive oil and olive leaf extract (OLE) due to their polyphenolic content. The main phenolic compound in OLE is glycosylated oleuropein (OLG), while the aglycon form of oleuropein (OLA) exists in much lower amounts. This work aimed to evaluate the effect of a low dose of OLG-rich OLE and the mechanism(s) that contributed to the observed beneficial effects against Aβ pathology in the homozygous 5xFAD mouse model. Mice were fed with OLE-enriched diet (695 μg/kg body weight/day) for 3 months, starting at 3 months old. Overall findings demonstrated that OLE reduced neuroinflammation by inhibiting the NF-κB pathway and suppressing the activation of NLRP3 inflammasomes and RAGE/HMGB1 pathways. In addition, OLE reduced total Aβ brain levels due to increased clearance and reduced production of Aβ and enhanced BBB integrity and function, which collectively improved the memory function. Thus, the consumption of OLE as a dietary supplement is expected to stop and/or slow the progression of AD.

https://pubs.acs.org...emneuro.2c00005

Edited by Phoebus, 20 November 2022 - 05:29 AM.

[Phoebus]

 

Published online 2022 Mar 25. doi: 10.3390/antiox11040629

PMCID: PMC9025619

PMID: 35453314

An Olive-Derived Extract 20% Rich in Hydroxytyrosol Prevents β-Amyloid Aggregation and Oxidative Stress, Two Features of Alzheimer Disease, via SKN-1/NRF2 and HSP-16.2 in Caenorhabditis elegans

Go to:

Abstract

Olive milling produces olive oil and different by-products, all of them very rich in different bioactive compounds like the phenolic alcohol hydroxytyrosol. The aim of the present study was to investigate the effects of an olive fruit extract 20% rich in hydroxytyrosol on the molecular mechanisms associated with Alzheimer disease features like Aβ- and tau- induced toxicity, as well as on oxidative stress in Caenorhabditis elegans. Moreover, characterization of the extracts, regarding the profile and content of phenolics, as well as total antioxidant ability, was investigated. The study of lethality, growth, pharyngeal pumping, and longevity in vivo demonstrated the lack of toxicity of the extract. One hundred μg/mL of extract treatment revealed prevention of oxidative stress and a delay in Aβ-induced paralysis related with a lower presence of Aβ aggregates. Indeed, the extract showed the ability to avoid a certain degree of proteotoxicity associated with aggregation of the tau protein. According to RNAi tests, SKN-1/NRF2 transcription factor and the overexpression of HSP-16.2 were mechanistically associated in the observed effects.

https://www.ncbi.nlm...les/PMC9025619/

 

So it looks like together Oleuropein and hydroxytyrosol work on both amyloid and tau plaques. 

[Phoebus]

An easier way to get HEPPS is at Amazon, from legit vendor RPI, 100 mg (or one kg).

 

https://www.amazon.com/dp/B01N5LTPCF

 

The RPI website offers 25 mg.  

 

Did you actually order this? 

 

I ordered it and it now says est deliery date is Jan 11th which seems really out there. Hopefully it actually gets delivered. 

[njurkovi]

An easier way to get HEPPS is at Amazon, from legit vendor RPI, 100 mg (or one kg).

 

https://www.amazon.com/dp/B01N5LTPCF

 

The RPI website offers 25 mg.  

 

Thanks for the link. I am not going to do the protocol right now, but I ordered it anyway because I tend to agree with Turnbuckle that this type of chemical will be harder and harder to get. The delivery date estimate: May 4-8 (??). Even for China this seems excessively long.
 

[Danniel]

It's been a while since anyone posted here. Are there any updates? Anyone can report if they tried the protocol, with which results and how were they measured?

[Meggo]

Is there any known vendor that ships HEPPS to EU countries?

Edited by Meggo, 12 July 2023 - 05:52 AM.

[rarefried]

Trigonelline sounds like a good candidate for this protocol.

 

Trigonelline recovers memory function in Alzheimer’s disease model mice: evidence of brain penetration and target molecule

[bullGenteel]

I wanted to share the update I saw amoung Turnbuckles other articles that were shared on his other threads.


Here is the update to Turnbuckle's protocol I found:

*********************************

Removing plaques
●      *HEPPS (200-500 mg initially, increasing to 1g)
●      *Taurine (6 g)
●      Nicotinamide (500 mg)
 
Detox
●      Hydroxytyrosol (100 mg)
●      Sulforaphane glucosinolate (50 mg)
●      Astaxanthin (24 mg)
●      *Vitamin C (500 mg)
 
Optional
●      *L-Serine (0.5-1 g), for memory
●      *Glutathione, reduced (0.5-1g), master antioxidant
●      *Melatonin (3-5 mg), only if taken at night
 
*Can be mixed into fruit juice
 
Notes
 
Taurine alone will not do the job. It requires HEPPS (also called EPPS). Do not use the similarly named HEPES. It is remarkably close to HEPPS chemically, but does the exact opposite. It compacts plaques rather than dissolving them.
 
For prevention or for early symptoms, 1 gram of HEPPS can be used initially. If there is a lot of plaque, removing too much at once can cause headaches and redeposition. So in that case, begin with a lower dose and increase it incrementally.

*********************


I believe Turnbucjle suggested a 3 month initial treatment or until all symptoms had abated.

Then a maintenance program of 2-4 times a month.

Or daily indefinitely if one has a lot of symptoms or suspect a heavy burden.

[bullGenteel]

I have had a moderate concussion I have been working to treat with alternative health practices, since traditional psychotrophic meds and traditional medicine did nothjng for me.

For about 8 months or so I have been doing a daily protocol similar to this update. I saw earlier in the thread, it was reccomended to do this protocol daily, indefinitely, if one suspects they have a high level of brain aggregates.

I do feel subjectively better off if I do this treatment regularly, compared to if I skipped it for a few days. It could be placebo. After I did three months I thought I felt modestely better in my day to day functioning. but I only did twice monthly maintenance, so I percieved I fell off from some of my gains.

It made some noticeable improvements for myself about a year and a half ago. I have gainned the most improvement from combining this protocol with experiments I did with the stem cell protocol created by turnbuckle.

For the last couple weeks I have added in tudca 500mg X2 day. I do the protocol at night with 5mg of melatonin. In morning I take second dose of tudca as well as more taurine, Oleuropein, vitamin C, gluthianine. Turnbuckle reccomended to take a second dose of taurine and some of the antioxidants around 4 to 6 hours to align with the half life of cerebral spinal fluid turnover to offer further protection, if you are just starting out with the protocol or have a higher level of aggregates.

The addition of tudca feels like a slight improvement to me, I read that tau protein may be more of a problem than plaque for cognitive functioning. I believe tudca was in an earlier iteration. But it can have some serious interactions wirh alcohol I believe.

From what I read it sounds like the lymphocytes that can absorb and process these aggregates into a more benign non toxic form, tend to slow down and become less efficient as we get older.

If one started this protocol sometime in middle age their brain would have less aggregates build up like a younger person's brain. I have no idea if that would be ideal or not. I remember mention that aggregates, atleast in the benign form may encapsulate viruses and such which may serve a purpose as we age.

[bullGenteel]

I would like to see if I could get some feedback on a slightly revised protocol for persons who are concerned about cancer. I have a friend whom is higher risk for cancer but may want to try some of the protocols such as this one.

I am only substituting some agents from the previous update to combine wirh some of the new agents. Some of updated agents seem to be anti-cancer, according my limited understanding from basic research. I am not science literate. I understand cancer prevention to be very complex and uncertain to even the experts.

I imagine it might be a good strategy to assume one has micro tumors or early states of cancer the body is currently dealing with, especially if one has a higher risk level.

So supplements to take even for an extended protocol, might want to ensure they are as anti-cancer as possible without any known cancer enhancers.

This is the slight edit I have proposed.

Removing plaques
●      *HEPPS (200-500 mg initially, increasing to 1g)
●      *Taurine (6 g)
●      Nicotinamide (500 mg)

************************************
Tudca (500mg)
I would add tudca, may be anti-cancer
************************************
 
Detox
●      Hydroxytyrosol (100 mg)
●      Sulforaphane glucosinolate (50 mg)
*****anti-cancer******
●      Astaxanthin (24 mg)
*****anti-cancer******
●      *Vitamin C (500 mg)
************************************
Sodium Ascorbate or regular
Vitamin C 3g
I believe I read vitamin C may be active as an Anti-oxidant at 3g or under per day.

• It could become a pro-oxidant at higher doses at 5g or more per dose. Pro-oxidants may be anti-cancer as some research proposes with high IV dose.
• Perhaps combine with this protocol to take high doses of sodium Ascorbate 2 or 3 times a day on an empty stomach as cancer prophylactic
□ Oleuropein (100 mg)
• Add back in Oleuropein from previous update for anti-cancer*******

Optional
●      *L-Serine (0.5-1 g), for memory
●      *Glutathione, reduced (0.5-1g), master antioxidant
●      *Melatonin (3-5 mg), only if taken at night
************************************
Remove optional agents except for melatonin. Gluthionine supposedly feeds cancer cells or lower ROS to allow tumors to thrive
************************************
************************************
□ Alpha-Lipolic-Acid 600mg
□ Hydroxycitrate 500mg
• I was wondering to add Alpha Lipolic acid to replace Gluthionine. I read that it can be recycled into gluthionine to some degree.
• But it is being researched as an anti-cancer agent combined with hydroxycitrate (Garcinia cambogia)

************

I would experiment with this new protocol, myself. The only agent I have a concern to prevent me is the alpha-lipolic acid. Since I have a mouthful of silver amalgams and ALA may chelate mercury and redeposit it thru the brain. My friend doesn't have any amalgams.

[EliotH]

A new study links low lithium in the brain to Alzheimer's. I've seen a couple of Youtube doctors recommend 5mg lithium orotate daily to stave off the disease. If I've got this right lithium binds to amyloid plaques (and maybe tau), thus depleting lithium which is needed for normal brain function. I haven't seen mentioned what happens to the lithium-bound plaque, whether it is disposed of or if it hangs around. I am adding lithium orotate to my daily supplements, in addition to TB's protocol.

 

https://hms.harvard....heimers-disease

[Mind]

A new study links low lithium in the brain to Alzheimer's. I've seen a couple of Youtube doctors recommend 5mg lithium orotate daily to stave off the disease. If I've got this right lithium binds to amyloid plaques (and maybe tau), thus depleting lithium which is needed for normal brain function. I haven't seen mentioned what happens to the lithium-bound plaque, whether it is disposed of or if it hangs around. I am adding lithium orotate to my daily supplements, in addition to TB's protocol.

 

https://hms.harvard....heimers-disease

 

There is a lot of good data over the course of the last few decades to support low-dose lithium supplementation. I have been taking it off an on for a few years. 

[bullGenteel]

It may have gleaned that researchers might think there are different causes of alzhiemers. I have heard that about autism/adhd/depression. It makes sense there are different variations of such illnesses. I just look at alzhiemers because I had a moderate brain injury. Doing basic research it appears similar in presentation ro alzhiemers.

I only ever found modest at best improvements using this plaque dissolving protocol. Noticeable, however I found more benefit from the mitochondria and stem cell protocols. But even the later's benefits stalled my improvement, when I know I have more room to improve.

So I did some research around four months ago. I found one paper that sounded very reasonable for a cause of alzhimers and my suffering as well. I think there are two kinds of avenues to explore as presented on this forum. Practical treatments that may make some real improvements. Other kind is exotic agents only aviable to researcher or that only work in lab settings or invitro.

There are some enzymes or proteins that may get shut off I skimmed a different study on that. There my be multiple systems that break down that can lead to alzhiemers. Those are really applicable at the moment in the real world. I'm just an everyday guy trying to look find practical solutions for myself. I am not capable of adding anything original to the work on this forum.

But back to the study I found. I discovered it aligned with questforlife's long thread on this website. He had a theory of aging that goes great degree of depth that already incorporated what was briefly introduced into he paper I found.

I've made some mistakes in incorporating questforlifes protocol. I am just finishing up my second one month round. I believe I have SCT variation of adhd, in addition to brain injury so my executive functioning and decision making can be skewed alot. Or my concentration needed for executive functioningmy attention maybe higher or average which I rely on in place of concentration. Consequently, I would have been further along if I hadn't messed somethingnup in my first round. I still feel progress is still on an upward trajectory. I kinda knew that, not unlike the stem cell treatments I did, that it may also take 4 rounds plus, to see some exceptional results. I might combine nsi-189, if I find a source because I believe the other protocol would be indusive to treating my variation of sct. I base off other research I revuewed from a frw years ago by another user who had sct.


The theory I am working from says that plaque tau and amyloid are the bad after effects of the real cause of alzhiemers. I am basing my interpretations off of reading researh and actual experimentation I have done on myself to gage what I belive is true. After my first round of the protocol of quest, I believe it was 2 weeks post that I got a bad headache. I am susceptible to the wishful thinking fallacy, it would be nice if one round of a protocol cured everything for myself. In the last 6 months I have mostly cut out my high dose regime of antioxidants. I usually dont feel my best if I am off antioxidant therapy or doing this plaque dissolving treatment. I have felt betrayed the last 6 .months. I also cutout ginkgo which I relied on as a crutch for cognitive functioning. Overall I am improving that was due to the stem cell therapy in conjuction with 5htp using Kelvin's warning to countering any negative effects of excitation. The headache went away within a day and a half of taking the this Alzhimer's protocol in combination of taking NAG. Plaque is suppose to come back in a prion type exponential growth, something along those lines. So definatly one treatment did not cure all the damage in my brain surprise surprise.

If quests protocol is successful I would gauge it by not having headaches come back and feel cognitively fully functioning, without ever using this plaque dissolvign protocol again. If you follow my litmus test for seeing if the new protocol will come close to a cure. I combine this new protocol with the stem cell as well as for maintenance I use this Alzhimer's protocol. Since the theory is that the cause of alziemwrs for some will also result in a build up off aggregates. In some cases it doesn't since autoposies of some alzhiemers pateients had average buildup of aggregates.

Fingers crossed, in recoguntion that I may always have to clear out plaques on an ongoing basis due to how serious my brian injury was, but I can hope. I have already felt like the improvement that plateaued with the combined stem cell aggregate removal maintenace has been reversed and i may continue to see see improvement.

I will report in the other thread or start my own to inform if I have have greater success I am hoping for. I appreciate the work in by this thread creator who made the biggest contributions and was responsible for bulk of this important work. I just blindly try to wrap my head around the gist of this work. In my limited understanding but perhaps based on my stumbling experimenting, I have to assume questforllife has made ground in his work. I don't understsnd much if it. There are risk I am taking ai can appreciate that. I concede his alternate direction is just as useful to my current situation. I'm a nobody so any improvement I can make will be thanks to other smart people here. I do worry about some of th risks as well.