4 replies to this topic

[necrobytez]

What does this REALLY mean?

 

Combined effects of alprazolam (Alp), a member of benzodiazepine group of drugs and caffeine on human cell lines, HeLa and THP1 were investigated in this study. Alp mediated cytotoxicity was enhanced while caffeine was present. The cell death was confirmed by observing morphological changes, LDH assay and membrane anisotropic study. Also such combined effects induced elevated level of ROS and depletion of GSH. The mechanism of cell death induced by simultaneous treatment of Alp and caffeine was associated with the calcium-mediated activation of mu-calpain, release of lysosomal protease cathepsin B, activation of PARP and cleavage of caspase 3. Our results indicate that, Alp alone induces apoptosis in human cells but in the presence of caffeine it augments necrosis in a well-regulated pathway. Thus our observations strongly suggest that, alprazolam and caffeine together produce severe cytotoxicity in human cell lines.

(http://www.ncbi.nlm....ubmed/19490937)

 

Is this in combination with xanax and coffee or can it happen days after you took xanax because it is still in your system. What does all this mean?

[Metagene]

It's in combination. Xanax has a short elimination half-life.

[YOLF]

Sounds like some interesting information. Perhaps readers would appreciate some more knowledge on the topic of PARP, cathepsin B, mu-calpain, and cleavage of caspase 3.

 

Given the hormetic effect of Methylene Blue, what might this information be used for? Or would it be too dangerous?

[medicineman]

What does this REALLY mean?

Combined effects of alprazolam (Alp), a member of benzodiazepine group of drugs and caffeine on human cell lines, HeLa and THP1 were investigated in this study. Alp mediated cytotoxicity was enhanced while caffeine was present. The cell death was confirmed by observing morphological changes, LDH assay and membrane anisotropic study. Also such combined effects induced elevated level of ROS and depletion of GSH. The mechanism of cell death induced by simultaneous treatment of Alp and caffeine was associated with the calcium-mediated activation of mu-calpain, release of lysosomal protease cathepsin B, activation of PARP and cleavage of caspase 3. Our results indicate that, Alp alone induces apoptosis in human cells but in the presence of caffeine it augments necrosis in a well-regulated pathway. Thus our observations strongly suggest that, alprazolam and caffeine together produce severe cytotoxicity in human cell lines.
(http://www.ncbi.nlm....ubmed/19490937)

Is this in combination with xanax and coffee or can it happen days after you took xanax because it is still in your system. What does all this mean?

please take studies on cells with a grain of salt. they may give us direction, but they shouldn't be taken too seriously. Xanax and caffeine are consumed simultaneously, by probably millions of people. these people have cells which have interstitial fluid and pumps and channels, and complexity beyond our science. hence they don't develop cytotoxic storms. the cells in culture are usually super sensitive and are naked. this is why many things are toxic to cell cultures which aren't problematic in physiological settings (calcium, cortisol) and vice versa (mptp)

I wouldn't let go of my coffee yet to be honest.

Edited by medicineman, 03 August 2014 - 10:33 AM.

[StevesPetRat]

To add to medicineman's point, both of those (HeLa and THP1) are highly ABNORMAL cancer cell lines.
But don't take Xanax. At least not more than a couple times a week.

Edited by StevesPetRat, 03 August 2014 - 10:58 AM.