mTor, JNK, AMPK, IGF-1/Calorie Restriction seem to be the four major routes that most supplements and drugs that have been shown to extend lifespan work through. A few months ago it was shown that Rapa, Rifa and Allantoin could consistenlty double the lifespan of flies and c.elegans. (Here is the full study: https://www.biorxiv....3205.full.pdf)
Most of us that have been watching longevity animal studies over the years have noticed that when researchers add two or more drugs that are known to extend lifespan independently, the end result is often a decrease in lifespan. The authors above noted that this phenomenon is likely caused by adding supplements with mechanical overlap/working through the same pathway which ends up negating each other's activity. Therefore, they identified these three supplements to work on all four of these pathways with minimal overlap.
So I am running Rapamycin at 1-2mg a week, Rifampicin at 150mg once every three days, and Allantoin through comfrey root with the harmful chemicals extracted. I have dropped Metformin, which has unimpressive results with Rapa in multiple studies. The only other supplements I will be taking are spermidine (which has been shown to have up to a 20% increase in mouse lifespan) and resveratrol (which works synergistically with spermidine on different pathways to promote autophagy). I rotate these into the regime after the Rapa is out of my system, because Rapa also promotes autophagy. I also rotate in Sulforphane Glucosinolate (Brocco Max) with NR to increase AMPK twice a week. I try to add in some glycine from time to time, which was shown to increase rodent lifespan up to 40% but, it may overlap with the other CR mimetics (Allantoin and Rapa) so I use it when those two are not in my system.
Thus far the only thing I have noticed is a surprising boost of energy about an hour after taking the Rifampicin and lasting several hours onward. I am going to be taking periodic liver functions tests to make sure the Rifa isn't causing any hepatotoxic problems. I will let you all know if anything comes of this, but I believe this regime is probably just about the best one available given the information we have thus far on longevity.