I liked the title of this one...running rats.....it looks like the dose was 100 mg/kg (see Methods section below)
Food Chem Toxicol. 2013 Feb 16. pii: S0278-6915(13)00099-9. doi: 10.1016/j.fct.2013.01.051. [Epub ahead of print]
Resveratrol enhances exercise training responses in rats selectively bred for high running performance.
Hart N, Sarga L, Csende Z, Koltai E, Koch LG, Britton SL, Davies KJ, Kouretas D, Wessner B, Radak Z.
Source
Research Institute of Sport Science, Semmelweis University, Budapest, Hungary.
Abstract
High capacity runner (HCR) rats have been developed by divergent artificial selection for treadmill endurance running capacity to explore an aerobic biology-disease connection. The beneficial effects of resveratrol supplementation have been demonstrated in endurance running and the antioxidant capacity of resveratrol is also demonstrated. In this study we examine whether 12 weeks of treadmill exercise training and/or resveratrol can enhance performance in HCR. Indeed, resveratrol increased aerobic performance and strength of upper limbs of these rats. Moreover, we have found that resveratrol activated the AMP-activated protein kinase, SIRT1, and mitochondrial transcription factor A (p<0.05). The changes in mitochondrial fission/fusion and Lon protease/HSP78 levels suggest that exercise training does not significantly induce damage of proteins. Moreover, neither exercise training nor resveratrol supplementation altered the content of protein carbonyls. Changes in the levels of forkhead transcription factor 1 and SIRT4 could suggest increased fat utilization and improved insulin sensitivity. These data indicate, that resveratrol supplementation enhances aerobic performance due to the activation of the AMPK-SIRT1-PGC-1α pathway.
Copyright © 2013 Elsevier Ltd. All rights reserved.
PMID: 23422033
2.2. Exercise protocol and resveratrol treatment
Twenty four HCR male rats, aged 13 months, were assigned to control HCR (HCR-C), trained HCR (HCR-Tr), resveratrol treated control HCR (HCR-Rsv), trained resveratrol treated HCR (HCR-TrRsv) groups (n = 6 rats per group). Control rats had access to the treadmill three times a week for 10 min with an electrical stimulator in place. Trained rats were introduced to treadmill running for 3 days, then for the next 2 weeks the running speed was set to 10 m/min, on a 5% incline for 30 min. The treadmill was equipped with a high pressure air pipe and electric grid to stimulate running.
In the following week, maximal oxygen uptake (VO2max) was measured on a motor driven treadmill (Columbus Inst. Columbus, Ohio) with a gradually increasing intensity. VO2max was measured for each animal, using three criteria: (i) no change in VO2 when speed was increased, (ii) rats no longer kept their position on the treadmill, and (iii) respiratory quotient (RQ = VCO2/VO2) > 1. Based on the level of VO2max, a treadmill speed corresponding to 60% VO2max was determined and used for daily training for one hr, five times per week. VO2max was measured every second week and running speed was adjusted accordingly. The total training period lasted 12 weeks. In addition, the forelimb strength of the animals was assessed weekly by using a gripping test as described by Marton et al. (Marton et al., 2010). Resveratrol supplementation (100 mg/kg, oral dosing) (Smith et al., 2009) was started 2 weeks before habitual treadmill running was introduced to the animals, and 4 weeks before the actual training started, therefore lasting 16 weeks. A dose response study on the toxicity of resveratrol revealed no toxic effects up to 1000 mg/kg in rats (Johnson et al., 2011).
