18 replies to this topic

[Marc P]

Hello,
I just got the RevG M98 micro Powder and I am mixing it with my whey protein shake in the morning.I was thinking about premixing the two powders and putting them in small single serving dark containers so its easy to mix up a shake in the morning after my workout.
My question is :
Will the resveraltrol loose potency if it sits with the whey mix to long?
It may be a silly question ,but I really dont know much about this stuff yet.
Thx Marc

[2tender]

Mornings are good. I wouldnt pre-mix them though.

[niner]

You could dispense the powders into two containers, and just not mix them. As long as the powders stay cool, dry, and dark there wouldn't be any problem there. To tell you the truth, I'm not convinced you couldn't safely mix them. You might try only doing a week's worth at a time.

[Anthony_Loera]

The beta-lactoglobulin is supposed to bind to the res and protect it from light and make it more soluble.

I think it's ok to pre-mix because of it and keep it in the fridge, just maybe not pre-mix for a whole month... more like a few days to a week. Overtime, the res that does not bind to the b-lactoglobulin will probably still settle at the bottom, so you will need to shake it up like most "tonics" out there.

Since you are just mixing for less than a day, I don't think there would be an issue.

A

[Brainbox]

Pre mixing with water or just the powders? I wouldn't premix whey with water with the goal to keep it longer than an hour. Regarding just the powders, I have no clue.

[Anthony_Loera]

I believe milk has a pH of 6.5-6.7 which is ok, while drinking water is about 7. But maybe not the best for pre-mixes for many days at a time.

From this 1996 study:
"Brent C. Trela and Andrew L. Waterhouse*
Department of Viticulture and Enology, University of California, Davis, California 95616"

http://pubs.acs.org/....1021/jf9504576

The conclusions state the following:

trans-Resveratrol should be handled with caution, but can be handled in the laboratory without stringent lighting precautions, whereas cis-resveratrol cannot.

Laboratory lighting conditions favor an equilibrium of about 91% cis-resveratrol.

Low pH causes cis-resveratrol isomerization to the trans isomer, a sterically more stable form. Some literature reports of cis-resveratrol are inaccurate and were addressed here.

It appears that at Ph of Pepsi (no, I personally prefer Pepsi over Coke.. sorry guys) has a Ph of about 3 or lower, and maybe good to "pre-mix" for those concerned with a very old batch of res that has turned into Cis-resveratrol... well, at least for those that don't mind the sugar high... :D


Another quote from the study:

cis-resveratrol degraded at pH 10.0 with a half-life of 63.7 h (Figure 7). cis-Resveratrol was stable at pH 7.0 (CV ) 0.7%), and less stable at pH 3.5 (CV ) 2.6%); however, at pH 1.0 isomerization to trans-resveratrol was efficient after 22.8 h (t1/2), 50% of the cis form had isomerized to the trans isomer nm).

and another...

Sample dry-down operations with rotary evaporation
were checked for resveratrol stability, and contrary to
Pezet et al. (1994) and McMurtrey et al. (1994), no
degradation of trans-resveratrol was observed after
evaporation of alcohol, confirmed by Jeandet even when
heated at 40 °C (unpublished results) or in buffers at
various pH values except for the pH 10.0 buffer.

of course, your gut has a Ph of about 1, so that may help as well.

Edited by Anthony_Loera, 24 July 2009 - 03:57 PM.

[2tender]

Anthony, is it okay to keep the M98 in the fridge? Thanks

[Marc P]

Anthony,
I am talking about pre-mixing the powder alone,not with the milk or water.

The beta-lactoglobulin is supposed to bind to the res and protect it from light and make it more soluble.

I think it's ok to pre-mix because of it and keep it in the fridge, just maybe not pre-mix for a whole month... more like a few days to a week. Overtime, the res that does not bind to the b-lactoglobulin will probably still settle at the bottom, so you will need to shake it up like most "tonics" out there.

Since you are just mixing for less than a day, I don't think there would be an issue.

A

Edited by Marc P, 24 July 2009 - 11:12 PM.

[maxwatt]

Anthony,
I am talking about pre-mixing the powder alone,not with the milk or water.

If the powders are dry, there should be no interaction on mixing them

[Anthony_Loera]

Hi Marc,

Sorry, when folks have talked about pre-mixing they always mentioned premixing the micronized res with an oil, milk, whey protein shake, etc.

I don't think there are issues with it being refrigerated, however like most loose powders, I would put it in the crisper.

Cheers
A

[niner]

No problem with keeping resveratrol in the refrigerator, as long as it's in an airtight container. When you want to get some out, though, you should let it warm to room temp before you open it, so you don't get water condensing on the inside of the container. This is too much of a hassle for things you use every day, imho. The only thing I keep in the refrigerator is in an aqueous solution. Everything else stays in a cabinet at room temp, in the dark.

[Marc P]

Hi Marc,

Sorry, when folks have talked about pre-mixing they always mentioned premixing the micronized res with an oil, milk, whey protein shake, etc.

I don't think there are issues with it being refrigerated, however like most loose powders, I would put it in the crisper.

Cheers
A

Anthony,
I said nothing about storing in the refrigerator.I just want to know if I can mix the powders and store them in a small (non transparent)container,like a small pill vile.That way I dont have to play chemist in the car and spill everything all over the place.
I will not store the containers in the car.I will keep them at home and bring one a day to my workout.
Marc

Edited by Marc P, 25 July 2009 - 08:39 AM.

[Anthony_Loera]

Marc,

I was answering your question and tender's question in one post. (see post #7)

Cheers
A

[2tender]

Thanks for posting that guys. Im keeping my pouch in a zip lock bag with a dessicant pack. I noticed a direct increase in energy and stamina upon my first day of adding 300 mgs of the powder to my existing 412 mgs (oral and transdermal combined) Resveratrol dose.

[zake]

Thanks for posting that guys. Im keeping my pouch in a zip lock bag with a dessicant pack. I noticed a direct increase in energy and stamina upon my first day of adding 300 mgs of the powder to my existing 412 mgs (oral and transdermal combined) Resveratrol dose.

Hi, I'm new,
Any suggestions about the micronized mixed into transdermal delivery?

[2tender]

Theres more to it than just dumping your powder into a carrier solution, although I dont know what the steps are. Whoever sold you the carrier should have info on how to do it. I buy my TD already mixed. There is a site that sells a carrier and has the steps posted, pm me for the addy.

[niner]

Any suggestions about the micronized mixed into transdermal delivery?

Some swear by it, but the science says it doesn't work.

[HUD199]

Hello,
I just got the RevG M98 micro Powder and I am mixing it with my whey protein shake in the morning.I was thinking about premixing the two powders and putting them in small single serving dark containers so its easy to mix up a shake in the morning after my workout.
My question is :
Will the resveraltrol loose potency if it sits with the whey mix to long?
It may be a silly question ,but I really dont know much about this stuff yet.
Thx Marc

Marc: I am a retired Chemical Engineer. I understand that Resveratrol is a very strong anti-oxidant. The longer you mix it, the more it will degrade. Aging studies on wine and liquid Resveratrol showed total loss of potency 3 days after opening the bottle. Tight recapping did not prevent loss of potency. Micro crystals should be more stable but condensation will activate oxidation process. For these reasons I chose to use an oil encapsulated, lab certified product called Rhythmatix.

My wife and I have been using Rhythmatix for a month. The Resveratrol is placed in fish oil, protected by Vit E, 75 mg CoQ10, and another bamboo extract, Unibex, anti-oxidant in a nitrogen purged capsule. Two small capsules yield a dose of 125 mg Resveratrol. It is sold in bottles of 120 caps, so 125 mg Resveratrol/day = $50/month retail. Rhythmatix has had 4 human clinical trials conducted on the mixture and is sold as a healthy heart supplement. No stomach problems. I am an active 64 yr old male, no meds, excellent blood numbers. I follow recommendations of The Metabolic Plan by Stephen Cherniske.

[niner]

Marc: I am a retired Chemical Engineer. I understand that Resveratrol is a very strong anti-oxidant. The longer you mix it, the more it will degrade. Aging studies on wine and liquid Resveratrol showed total loss of potency 3 days after opening the bottle. Tight recapping did not prevent loss of potency. Micro crystals should be more stable but condensation will activate oxidation process. For these reasons I chose to use an oil encapsulated, lab certified product called Rhythmatix.

My wife and I have been using Rhythmatix for a month. The Resveratrol is placed in fish oil, protected by Vit E, 75 mg CoQ10, and another bamboo extract, Unibex, anti-oxidant in a nitrogen purged capsule. Two small capsules yield a dose of 125 mg Resveratrol. It is sold in bottles of 120 caps, so 125 mg Resveratrol/day = $50/month retail. Rhythmatix has had 4 human clinical trials conducted on the mixture and is sold as a healthy heart supplement. No stomach problems. I am an active 64 yr old male, no meds, excellent blood numbers. I follow recommendations of The Metabolic Plan by Stephen Cherniske.

I would say that resveratrol is an antioxidant, but it's not all that strong; not to the point that it's wildly unstable. The wine and liquid resveratrol studies are demonstrating that stability problems are much more prevalent in solution than in the solid phase. I agree with you about the condensation problem; I store my powdered supplements at room temperature for that very reason. Rhythmatix sounds interesting; I've not heard of it before. Considering it strictly as a resveratrol source, it's pretty expensive in terms of grams of resveratrol per dollar, relative to other sources of sophisticated resveratrol formulations.