1079 replies to this topic

[QuestforLife]

I thought about trying Rapamycin. 

 

I don't take rapamycin anymore. I tried it for 2 years at a low dose and then decided to stop. I then tried everolimus and rapamycin at higher doses. I'd say they were harmful at that dose. 

I find mTOR inhibitors thin my hair and make me feel worse. I also found this with astaxanthin. 

[QuestforLife]

I am a little leary of over using stem cell agents. I believe you posted a mouse study where mice who dipped into bone marrow stem cells had repaired injuries but had 30% reduction in lifespan.

 

I am still undecided on this. 

 

I feel that humans may have telomerase positive stem cells, it is just they are very few in number and they haven't been widely identified yet. If this is the case then we probably can just stimulate them for benefits without worrying about running out. But they clearly fail with age in any case, so we need to work out why this happens. Hint, they probably get stuck in the connective tissue. 

[JamesPaul]

Have you considered nicotinic acid, niacin?   Antiaging youtuber Michael Lustgarten found that around 60mg iirc of niacin achieved similar nad test results to 1000mg of nmn, iirc.  50mg, did not raise nad.  Also some research suggests nmn and nr are converted into niacin in the gut.

 

Given that info, I had started taking 100mg niacin, a bit higher but just to be on the safe side.   It is very cheap.   Though I've not tested my nad yet.

 

From what I understand there is a sweetspot to nad+ levels.  Too high is not good.   Regards nmn I think donotage is a good brand for that.  I also think that nmnh is also good from doublewood.  But nmnh is said to be stronger than nmn at raising nad+ levels, and I'm not sure what the comparable to 1000mg nmn dose of nmnh would be.

Dr. Greger discusses NMNH and NRH starting at 4:00 in the video below. In vitro, NMNH raises NAD levels 10 times as much as NMN. He also said that because of the extreme potency of NRH in raising NAD levels (50 times as potent as NR), NRH was shown to have detrimental effects on human liver cells in vitro due to accumulation of free radicals. NRH also had pro-inflammatory effects on human immune cells in vitro. Because NMNH is converted into NRH in order to enter cells, Dr. Greger said that NMNH might share the potentially deleterious effects of NRH, although this has yet to be tested.”

 

“Lesser-Known NAD+-Boosting Supplements: Tryptophan, NADH, NMNH, and NRH".

[bullGenteel]

Thanks for getting back to me on my posts.

After yesterday I was going to post that I had been premature in claiming my 10 year long plus, swollen ankle is on its way to mending. I didn't sleep well the night previous and so yesterday my ankle looked almost the same as a few weeks ago.

I think sleep must be very important if I am hoping for rejuvenation. I am in my early forties, but I don't necessarily feel it or look it. I feel mk-677 does help for my age and recovery and aids my overall well being. I do feel like my exercise had improved a lot just with mk-677 before even doing stem cell protocols, even back a year ago. I felt like in this last two weeks, the few times I exercised, when on a good night's sleep, I feel my exercise is closer to how I felt 10 years or more ago. Or I could be moving in that direction, it's easy to let enthusiasm sway myself. My ankle and older broken arm doesn't feel much in the way of soreness the day after. Before It would be noticely more swollen and strained.

Today, I did take ginseng on an empty stomach this morning. 8 hours later my ankle looks the closest in size it has looked to my non-injured ankle. That's how it looked a few days before yesterday.

I took curcumin for 8 or 9 months and ginseng and the most it improved was like 50% reduction. It stayed about the same for a year and a half. Than the stem cell(turnbuckle) lowered it like another 25% over a few months. It has been stuck at 25% bigger for the last few months. There is definitely some improvement from the herbal rediferentiation protocol wi4hin thisnthread I am doing now. It has never gone down in size over a day nor in 3 weeks.

Yesterday, I was just thinking it might be a good idea to combine this protocol with some NGF and remyleniation agents. I added alcar today and ginseng I might take a few days a week. Ginseng can be used for telomeres I read in here. I might add more lecithin and Choline.

I have taken icariin on and off for 8 months and never really noticed much. I noticed a lot of improvement in cognition from agamatine which is suppose to help wirh neurogensis as well. I just caught on a post here that icariin absorption can be boosted 60% from a lactase inhibitor. It's mentioned on examine.com too. I may have to order that from bulk supplements.

[bullGenteel]

I also sort of felt the greatest cognitive boost from ginseng, but it is estrogenic. Perhaps the same problem with curcumin or it just is too dopenergic for my temperament. However, No long term permanent improvements. Seems like the strm cell protocols and hopefully thisnprotocol will gove some permanent improvements.

I would say reuteri yougart restores a lot of youthful energy and revovery for myself the last 3 years. It can increase prolactin from a study on rats I may have concluded. That seems to be countered by curcumin. Or in place of curcumin than with vitamin e or high dose chondriton or ginkgo and vitamin e. I could feel really down than feel better after taking a high dose of vitamin E, especially after sexual activity.

It hasnt been a problem for months perhaps collagen on an empty stomach really does helps lower serotonin. I don't remember the details or even why I think this, but I was certain in my conclusion that SSRI's also raise prolactin. I dont keep notes so I dont know if my conclusion was valid or not. I cut out ginkgo few months ago. It does increase histamine.

I have no allergies, but if I am taking yougart regularly, I will have to blow my nose often. I tried combining it with yougart strains but perhaps only histamine neutral strains, rather than anti-histamine. I think there is another strain, the salivarius that may be more of a true anit-histamine. It's not a big problem for me so I haven't tried to fix it. I use to take high dose Vitamin C like 18g, but still had slightly raised histamine.

Edited by bullGenteel, 14 May 2025 - 03:17 AM.

[bullGenteel]

Being ignorant, I vaguely wonder if rapamycin could help if combined wirh this protocol and some neurogeneiss agents with wild hoping. From what little I may have figured from this site is that testing one agent on its own won't give much ROI but might be a game changer when combined effectively from smart people figuring out protocols thru research and experimentation.

Yesterday, I was just thinking it might be a good idea to combine this protocol with some NGF and remyleniation agents. I added alcar today and ginseng I might take a few days a week. Ginseng can be used for telomeres I read in here. I might add more lecithin and Choline.

I have taken icariin on and off for 8 months and never really noticed much. I noticed a lot of improvement in cognition from agamatine which is suppose to help wirh neurogensis as well. I just caught on a post here that icariin absorption can be boosted 60% from a lactase inhibitor. It's mentioned on examine.com too. I may have to order that from bulk supplements.

Edited by bullGenteel, 14 May 2025 - 03:24 AM.

[bullGenteel]

I never really found much benefit from fasting. Not to cure my concussion or injuries. It probably pushed me some in right direction. I did countless 10 day fasts, a 21 day fast and even 30 days. I felt kinda stupid on reflection, some time after. I believe by not first addressing sencience cells and or brain aggregates, then there wasn't much the fasting could do in way of real lasting improvements.

I wonder if since long term fasts are supposed to release a lot of toxins from the deep tissue then it could unlock stem cells maybe just a thought. I want to say my weight dropped to the lowest its ever been but I don't have a great memory and don't keep records. I think it was on the very lowest end that can still be considered safe. I remeber a member here who is my height and practices CR who reported being 10 LLBS less than the lowest weight I went to last spring. I imagine any unlocked stem cells i may have exposed were wasted because I didn't use fusion or stem cell protocols after my extended fasts.
I am thinking about trying the sartin and statin protocol, sometime in future after this herbal version. It appears to be working. I thought you wrote you had more greater postive effects from the medications.

I might want to look up your version of the stem cell protocol. Is it in this thread? Would the telomere positive stem cells also be the unselfish stem cells. I've never had a great memory. But I also don't think I was able to attempt to wrap my head around that theory. I may reread some of that at a later time.

[QuestforLife]

I never really found much benefit from fasting. Not to cure my concussion or injuries. It probably pushed me some in right direction. I did countless 10 day fasts, a 21 day fast and even 30 days. I felt kinda stupid on reflection, some time after. I believe by not first addressing sencience cells and or brain aggregates, then there wasn't much the fasting could do in way of real lasting improvements.

 

 

I don't think you've been stupid at all: I expect fasting has done you a lot of good. All anti aging pathways go through calorie restriction after all! 

 

But I do think we can achieve more if we stimulate stem cells in the right way. But metabolic health comes first, in my opinion. 

 

Dr. Greger discusses NMNH and NRH starting at 4:00 in the video below. 

 

I haven't tried NMNH. But I've tried many other NAD+ boosting supplements, and these experiments are outlined in this thread.

 

I have now come to the view that the best of the lot is...niacin. 

 

I take it everyday with lunch and dinner. There is no need for any of the others. 

[QuestforLife]

Being ignorant, I vaguely wonder if rapamycin could help if combined wirh this protocol and some neurogeneiss agents with wild hoping. 

 

Maybe. But its half life is loong. Difficult to time it right because of that. You'd be better off doing a (short) fast combined with fusion supplements and C60 (take this later after you are really into the fat burning) to really encourage that fat burning and stem cell renewal. There's other supplements that can help here, too many to list. For example I've heard blueberries are great for stem cells. But you wouldn't want to take in too much sugar, so an extract would be better. Not tried that myself yet. Then when you re-feed, really flip the switch and encourage growth and repair of all your tissues.

[bullGenteel]

Thanks for the suggestions. I was wondering about the high and low dose of rapamycin, I read. I couldn't really procure that much rapamycin, so good to know the low dose was best in your experiments.

I may get around to thinking about buying some statins and I can purchase rapamycin from same source. I might combine weekly rapamycin with that protocol. It might be nice to see if the rapamycin, following a fast might give me more enhanced positive results again from c60. I did start to get diminishing results after 5 cycles or so.

I guess that may be common and you need to find a way around fat burning homeostasis if I caught the gist of what you mentioned on another thread. For my experiments, I thought it was because I wasn't addressing sencient cells I acquired out of a serious accident. I am wondering if I perhaps added in an mtor enhancer during fusion days at the time I satrted getting less postive effects or even some mild reversal of progress.

I added the same substance in now. I may do some more reading and experiment by dropping mk-677 as a mtor agent, if it is one. I read it can activate mtor in hypothalmus and hippocampus. Ghrelin modulation from mk-677 might also upregulate mtor thru orexin. I can experiment and see if my ankle feels better without using it.

It is also possible I thought that I got excitology from taking too high of dose of 5htp that may have damaged fine neurons. From another users research i found 5g of collagen resolved any speed up or excitology like effects I got from c60 and 5htp.

I saw an article, by chance that might be interesting for fusion. I'll read up more on it, or it will be easy enough to experiment with, I think. I will have to read up some more.

I am not sure if ginseng inhibits mtor or not, or if it's has compounds that act in opposite ways on certain pathways

Edited by bullGenteel, 15 May 2025 - 07:44 AM.

[bullGenteel]

I did some more research into your Sirtin/statin protocols this last week or so. Considering I'm coming up to the end of my trial it is belateedly fitting. I thought I understood enough from reading a lot here, but I struggle with details and even organizing my attempts at protocols. I guess some short comings at logical thinking and some effects from a moderate concussion are at play. One mistake I did was to upregulate mtor because I didn't take time to fully understand your protocol in my layman level. Thus may have hindered my attmepts to shrink cells to allow for multi potent cells to later redifferenetiate.

I was half winging it and contemplating ending the protocol now, even though I may have only corrected this process the last week or so and It's been atleast a month. I have had my wild ideas for what to do at the end of this protocol with c60. I think I will do a 3 day fast than take c60 as I usually do.

I saw a post about your c60 protocol, I believe. I was thinking of doing something similar. Your protocol and another thread of your's sounded similar to another update to a protocol I have tried of another users protocol. I actually saw in the first paper you have listed for your protocol 2 agents that I was thinking of using because one of them is listed in the other thread and I guessed at the inverse synergistic agent that is mentioned also.

But I don't have a supply of the one agent in question. I used an earlier iteration of the other users' protocol with different agents. I was thinking of using that with c60 because it is all I have. For this other user's protocol, on an off chance, I saw a paper that lists a third agent I use regularly that may downregulate glycoisis, which is one of the hinderances in the earlier iteration of the other protocol I have used wirh some success. Perhaps that is why it seemd to work well for myself. I haven't reviewed the paper about this third agent agmatine and it may blocck glycolysis to an extent. I do know it would be beyond my capabilities to decipher. I don't know if I could just take it at best guess that it will somehow synergize. with. Agmantine may lessen glycolsis, but, considering I have no scientific understanding then again it might not be significant.

I nevwr used the other user's protocol with the c6p protocl, but I did a few days after having done both a 3 day fast and a few days of c60 protocol. I remember 5 years ago when I did my first 3 day fast I noticed regular colors at night almost look like neon. We'll street lights did and regular colors looked intense. I wonder if it is more dramatic an effect for myself because my baseline color perception may be low as a result of a brain injury.

Around the time after I had done did my second 10 day fast I noticed that the pronounced color vision became more or less permanent at night. I also forget when I stopped needing glasses to drive at night. I needed glasses since my mid 20's just for night driving. I haven't used glasses for years and my eye sight has perhaps improved even further lately. Sort of a a small victory considering having post concussion syndrome sucks. But it can be bearable, even just with treating inflammation but it seems to be improving greatly with my pursuits the last 8 months or so.

The first time I did this other fusion protocol preceding c60 at around 6 months ago, I noticed that colors for the first time were more enhanced during the day. It only lasted a few hours if I recall.

This morning I took one buckthorn berry pill for the first time in this regeneration protocol of yours. After taking some low dose nmn, I noticed that colors look more enhanced again. I also took the roc/loc inhibitors. It is subtle I don't know if it was after some time and not an effect from low dose NMN.

I wonder if damage around visual processing or maybe the cones of the eyes are really sensitive to enchantment from stem cell activity.

I do have a memory from around 3 years of age at the holidays. I remember at night all the colors and decorations looked even more enhanced and also the next morning. I've always been a more visual person. This effect isn't as strong as when I was a child. I'm sure everything is more potentiated as a kid with functioning at a more pristone, optimal level.

[bullGenteel]

I think I will try a 3 day fast than try your c60 protocol, perhaps wi4h one buckthorn berry tab. If I recall in reading here, you may have a 4 hour window to take the c60 at the end of that to push the fat burning and fusion. My bottle of c60 has been frozen, but I have taken it out in evenings in low light maybe 6 times or so. I wish I had taken it out in complete darkness and measured a teaspoon by feel. That would have been beyond my consideration to attempt before. I'm not the most ambitious functional at times. Hopefully it is fine or I may wait for a new bottle.

Your version of c60 protocol seems a bit easier than the one I devised or inspired from on the other users thread. I might wait till I have the updated protocol agents. I don't know if I am comfortable using agmatine. It probably isn't needed though, since the other agents give me a boost for a few hours.

I read from Longo that stem cells can be active for a few weeks, in my experience it has seemed like 2 weeks. With your protocol I may take mk-677 for GH promotion and then cut out inhibitors or replace with promoters for differentiation and growth. If I followed your thread corectly, than I may hope for some regneeration over the next couple weeks. Keeping in mind I was a little lost in my attempt this last month.

[bullGenteel]

I'm away from my computer at the moment, but I did see a paper about a combination of rapamycin and another agent. I saw the second agent is for purchase for a few hundred dollars. I was a little more fuzzy at the time of reading this. It seemed to say it was more effective than rapamycin alone. The second agent acted on mtor2 to give more benefits, if I recall. I don't think I'm at a level to understand but I can reread it and also post it later.

A couple months ago I was reading a bit about cancer and Alzheimers. Since Alzhimers is similar in presentation to brain injury. One article was explaining how a researcher belives that cells and neurons being inefficient at disposing of waste is what leads to neuron death and the release and build up of aggregates that lysossomes cannot keep pace with.

This seemed intriguing since I found some benefit from aggregate reducing treatments, but it was not as great degree of benefit compared to stem cell enhancements.

I was going to question you about this paper, since you mentioend rapamycin greatest benefit may be making cells smaller and more efficient. I re-read alot of your thread and you already went into a lot of detail about how your regeneration protocol can also make cells smaller and more stem like and effcient at clearing out waste. Except neurons are not replaced very often and you mentioned division is when waste can be removed effectively. Still, it seems that rapamycin and your protocol may be helpful to brain damage and alzhiemers.

Cells can die or become sencient after damage. Perhaps if they are smaller that may be significant enough to allow the proper removal of waste. Perhaps combined with mitochondria optimization and atp or nad+ to make cells more efficient. I read some posts in this thread where you summarized a lot of this already.

There's a lot of theories and it likely could be an amalgamation of a lot of things going wrong to cause alzhimers or aging in general. Similar to umbrella terms like ADHD or even brain injury that may have multiple underpinnings.

[QuestforLife]

I think I will try a 3 day fast than try your c60 protocol, perhaps wi4h one buckthorn berry tab. If I recall in reading here, you may have a 4 hour window to take the c60 at the end of that to push the fat burning and fusion. My bottle of c60 has been frozen, but I have taken it out in evenings in low light maybe 6 times or so. I wish I had taken it out in complete darkness and measured a teaspoon by feel. That would have been beyond my consideration to attempt before. I'm not the most ambitious functional at times. Hopefully it is fine or I may wait for a new bottle.

Your version of c60 protocol seems a bit easier than the one I devised or inspired from on the other users thread. I might wait till I have the updated protocol agents. I don't know if I am comfortable using agmatine. It probably isn't needed though, since the other agents give me a boost for a few hours.

I read from Longo that stem cells can be active for a few weeks, in my experience it has seemed like 2 weeks. With your protocol I may take mk-677 for GH promotion and then cut out inhibitors or replace with promoters for differentiation and growth. If I followed your thread corectly, than I may hope for some regneeration over the next couple weeks. Keeping in mind I was a little lost in my attempt this last month.

 

I came up with that a long time ago. Do you own research before starting on a new protocol to make sure it is right for you. 

 

I do think there is some merit in looking at products that increase stem cell numbers - other than C60. 

 

When I did experiments using Stem Enhance (which is blue algae, mainly), the main thing I noticed was fatigue. 

 

You need to think about supplementing all the things new cells will need. I'd recommend TMG or Methionine and Glutamine to start with. Perhaps some other things too like arginine and leucine. This goes for people using the standard TB C60 protocol as well. 

 

I currently am not doing anything like blue algae or buckthorn, etc. But I am considering trying blueberries (freeze dried or an extract) to add to what I am already doing.  

 

This thread is mainly research into things that may move the needle meaningfully on telomeres or stem cells. I am not big on pushing my own protocols but I do share what I am doing from time to time and any benefits or pitfalls I find. 

[bullGenteel]

Thanks for the feedback, I appreciate how this thread seems to go into greater details about a lot of different cellular processes. A lot of the protocols covered here seem like could be applied to more than just anti aging regime.

I know I wouldn't really reccomend or advise anyone to follow my example, since I'm a little scatterbrained in trying to feel my way forward. I was thinking if I get results I'm looking for, I may post a thread. To pay it forward because I found a lot of inspiration and great protocols on this website that have actually made real life improvements in my condition compared to what is available in medical establishments. Here's hoping I do more good than harm on my journey. I think it would have to be a personal decision, if the potential risks down the road are worth the greater quality of life I feel I may gain from these protocols. I do appreciate them being developed and shared.

I do feel an improved sense of well being. I improved my sleep schedule the last few nights, which probably is great importance if I am attempting to repair or rejuvinate some old injuries. I noticed less dull pain in my ankle. I can actually see the outline of a tendon, running thru the most swollen area as well as a vein, which I couldn't see before.

I do get more HD vision with brighter colors and feel a bit sharper the last couple days too. I read in a CILTEP threads that people got those effects from that protocol and maybe from forskolin alone they attributed gains. They reccomended pairing it with a choline source because it is an acetylcholinergic. I just added regular choline biturate, to keep it simple.

[bullGenteel]

I decided not to jump into a 3 day fast, just yet. I slept on it and decided I may do a fast using the stem cell protocol supplements in thier entirety, except for c60. I got results before that way, it would relieve my concerns about my bottle of c60 being 7 months old. I read about mice study researchers being worriid about light induced toxins. Also, another ussr posted a paper thst stated that even ambient light can cause toxins to be produced in c60.

I think I will order a small bottle of carbon60oliveoil.com brand for next week. Then I can go in a dark closet and portion it out to 3 small metal, light-tight bottles, to be a bit stricter in safe storage practice.

I also reviewed this thread in more detail again. I feel I wasn't following the guidelines very effectively. I didn't really cut down enough on mtor, and also maybe sirt 4. I only took half the reccomended dose of Forskolin and artichoke extract for camp activation. I need to work on having more attention to details.

So I decided to push it another week or so with the protocol stage of trying to shrink cells and adapt them to a more stem like state. Then I will add the other agents you suggested to go for growth and cell division. I do wonder if I try greatly cut down any growth, at this stage, maybe that will give relief from lethargy, but it's just a random guess. I only felt tired last night, in a similar way I felt doing stem cell protocol. Maybe, because I had skipped reversatrol yesterday and had more growth activity as a result. I am only using a low dose of reversatrol maybe 10-20mg with some copper used a few times thru out the day. I don't know if that is enough to activate sirt-4 to stop growth to trade off for reduction of mote dedifferentistion. You stated reversatrol is seen as a poison, so the body in response halts growth.

I have read that sometimes stem cell may by some kind of method release catalysts to aid cells in injured sites to repair. If that's true they do not necessarily need to replace cells, so perhaps by taking buckthorn berry stem releasing agent it may still somehow move the process along of inducing cells to a more stem like state. I am only planning to take the buckthorn berry supplement a total of 4 times on tail end of this protocol, every other day. I wasn't sure if it was needed while the cells are being primed to return to a more stem like stage. I am a little concerned about stimulating bone marrow stem cells too much.

[Castiel]

Regards stem cells in adults from my understanding there are telomerase positive ones in tissues like skin but the rate of activity is not high enough to stop eventual stop of cell division viability.

As for fasting ive heard 3 days of fasting triggers stem cell renewal. Dark chocolate flavonols do as well.

Btw, touchstone essentials finally released the telomere lengthening compound based on Dr. Bill Andrews research. The product is called telo vital. I recognize a few of the ingredients in the product but some Id not heard of. These are the strongest telomere lengthening ingredients Andrews has found through lab tests iirc
Regards stem cells in adults from my understanding there are telomerase positive ones in tissues like skin but the rate of activity is not high enough to stop eventual stop of cell division viability.

As for fasting ive heard 3 days of fasting triggers stem cell renewal. Dark chocolate flavonols do as well.

Btw, touchstone essentials finally released the telomere lengthening compound based on Dr. Bill Andrews research. The product is called telo vital. I recognize a few of the ingredients in the product but some Id not heard of. These are the strongest telomere lengthening ingredients Andrews has found through lab tests iirc
Regards stem cells in adults from my understanding there are telomerase positive ones in tissues like skin but the rate of activity is not high enough to stop eventual stop of cell division viability.

As for fasting ive heard 3 days of fasting triggers stem cell renewal. Dark chocolate flavonols do as well.

Btw, touchstone essentials finally released the telomere lengthening compound based on Dr. Bill Andrews research. The product is called telo vital. I recognize a few of the ingredients in the product but some Id not heard of. These are the strongest telomere lengthening ingredients Andrews has found through lab tests iirc

[Advocatus Diaboli]

Consumer Reports suggests that dark chocolate shouldn't be eaten on a long-term basis:

 

 

Published December 15, 2022·Updated October 25, 2023

 

"But there’s a dark side to this “healthier” chocolate. Research has found that some dark chocolate bars contain cadmium and lead—two heavy metals linked to a host of health problems in children and adults. 

 

The chocolate industry has been grappling with ways to lower those levels. To see how much of a risk these favorite treats pose, Consumer Reports scientists recently measured the amount of heavy metals in 28 dark chocolate bars. They detected cadmium and lead in all of them.

 

CR tested a mix of brands, including smaller ones, such as Alter Eco and Mast, and more familiar ones, like Dove and Ghirardelli. 

 

For 23 of the bars, eating just an ounce a day would put an adult over a level that public health authorities and CR’s experts say may be harmful for at least one of those heavy metals. Five of the bars were above those levels for both cadmium and lead.

 

Consistent, long-term exposure to even small amounts of heavy metals can lead to a variety of health problems.

 

Even if you aren’t a frequent consumer of chocolate, lead and cadmium can still be a concern. It can be found in many other foods—such as sweet potatoes, spinach, and carrots—and small amounts from multiple sources can add up to dangerous levels. That’s why it’s important to limit exposure when you can.

 

Calculating the exact amount of dark chocolate that’s risky to eat is complicated. That’s because heavy metal levels can vary, people have different risk levels, and chocolate is just one potential source of heavy metal exposure. But experts say that by being mindful of the risks, you can still enjoy dark chocolate while minimizing the potential harms."

Edited by Advocatus Diaboli, 08 June 2025 - 08:54 PM.

[pamojja]

But experts say that by being mindful of the risks, you can still enjoy dark chocolate while minimizing the potential harms."

 

I've been eaten 20g 100% cocoa powder and about 20g at least 85% dark chocolate for a decade. Along with tons of Ayurvedic herbs, known to be contaminated by heavy metals too.

 

Fur assurance, I did for 8 consecutive years hair tissue mineral analysis. The only heavy metal which rose for about 2 years above normal was uranium, coincidentally after Fukushima.

 

So do test, don't guess.
 

[Castiel]

Two things

first investigating some of the ingredients in telo vital got comments saying these ingredients require cycling or off periods.

Second there are cocoa extracts like cocoa via with undetectable heavy metals, also some other cocoa powder brands claim to test for heavy metals. But seeing the consumer lab independent results should be good and as others mentioned personal tests sound good too

[Alessandro]

I don't think you've been stupid at all: I expect fasting has done you a lot of good. All anti aging pathways go through calorie restriction after all! 

 

But I do think we can achieve more if we stimulate stem cells in the right way. But metabolic health comes first, in my opinion. 

 

I haven't tried NMNH. But I've tried many other NAD+ boosting supplements, and these experiments are outlined in this thread.

 

I have now come to the view that the best of the lot is...niacin. 

 

I take it everyday with lunch and dinner. There is no need for any of the others. 

KL-1333

[Alessandro]

Inhibition of Rho-associated kinases suppresses cardiac myofibroblast function in engineered connective and heart muscle tissues

 

https://www.jmmc-onl...1259-8/fulltext

 

An interesting paper on using ROCK inhibitors on an engineered connective tissue model. Shows ROCK inhibition blocks the effects of tgf-b on tissue contraction and stiffness.

And…

This may explain why my protocol seems to be causing me some irregular heartbeats. I eliminated oxytocin as the cause – it causes short lived flushing, drop in heart rate variability and a rise in heart rate, but doesn’t cause the heart to jump about erratically.
More generally the use of 3D substrates in this study, rather than an overly stiff 2D petri dish echoes earlier comments I made about the inappropriateness of 2D cell cultures for studying the body, and also suggests ROCK inhibitors will have a stronger (or at least different) effect in the body that in a dish.

This suggests that inhibiting tgf-b and ROCK together is worthwhile, and that a ROCK inhibitor in a high tgf-b environment might be less effective.

Also mentioned in the paper, Fausidil is a partial PKA inhibitor, meaning in too high a concentration it will inhibit cAMP – the opposite of what we want to do.  I have no information on whether phloretin would do the same, it seems unlikely at my dose and the boost to alertness I have got from forskolin, but it is something to bear in mind. ROCK inhibitors also appear to be ATP competitors, so side effects are almost guaranteed if you take too much.

In any case I intend to terminate my herbal protocol this week, give a final report on it, and do my blood tests. I am doing a DNAge methylation test as well as a standalone CRP test.

https://pubmed.ncbi....h.gov/15588713/ What do you think? Could prevent or cause arrythmias like in your case?

Edited by Alessandro, Yesterday, 04:38 PM.

[Advocatus Diaboli]

Re: post #1069

 

"I've been eaten 20g 100% cocoa powder and about 20g at least 85% dark chocolate for a decade."

 

Was that a daily ingestion, pamojja?

 

"The only heavy metal which rose for about 2 years above normal was uranium, coincidentally after Fukushima."

 

That's interesting, pamojja, considering the following:

 

Query to Perplexity AI:

 

"Was any uranium released into the atmosphere or in food products or by other means from Fukushima that could have ended up as detectable levels in hair tissue mineral analysis in humans in Austria."

 

Answer:

 

"Based on current scientific reports and official data, there is no evidence that uranium released from the Fukushima accident reached Austria at levels detectable by hair tissue mineral analysis in humans.

Key points:

• The primary radioactive elements released from Fukushima were iodine-131 and cesium-134/137, with smaller amounts of strontium and plutonium also detected5146. These were the nuclides most closely monitored for environmental and health impacts.

• Reports and monitoring by international agencies, including the IAEA and NOAA, do not list uranium as a significant airborne or foodborne contaminant from Fukushima reaching distant countries such as Austria568.

• The vast majority of radioactive releases into the atmosphere and ocean were iodine and cesium isotopes, which are much more volatile and mobile than uranium561. Uranium is heavy, less volatile, and far less likely to travel long distances as an airborne contaminant.

• No official or peer-reviewed sources report detectable uranium from Fukushima in European populations or the environment, nor in food products exported to Europe568.

• The expert consensus is that the health risks outside Japan were primarily from iodine and cesium, not uranium5.

Conclusion:
While trace amounts of uranium may have been released locally at Fukushima, there is no credible evidence or scientific report indicating that uranium from Fukushima could have reached Austria in quantities detectable by hair tissue mineral analysis in humans. The radioactive contamination of concern for distant populations was overwhelmingly due to iodine and cesium, not uranium568."

 

What is your response?

 

Re: post# 1070

 

"Second there are cocoa extracts like cocoa via (sic) with undetectable heavy metals, also some other cocoa powder brands claim to test for heavy metals."

 

Castiel, Here is what DeepSeek AI says about CocoaVia:

1. CocoaVia® (by Mars)

   • Claims: Third-party tested for heavy metals; their processing reduces contaminants.

   • Reality: Independent tests (e.g., Consumer Reports) still detected trace metals, but below Prop 65 limits.  (my emphasis)

Unfortunately, self testing is ex post facto.

[QuestforLife]

Regards stem cells in adults from my understanding there are telomerase positive ones in tissues like skin but the rate of activity is not high enough to stop eventual stop of cell division viability.



 

 

That is what they say. But is it true?

 

 Btw, touchstone essentials finally released the telomere lengthening compound based on Dr. Bill Andrews research. The product is called telo vital. I recognize a few of the ingredients in the product but some Id not heard of. These are the strongest telomere lengthening ingredients Andrews has found through lab tests iirc

 

Ah that's interesting. Kinda of suprising they just show the ingredients for all to see. I remember Bill Andrews had a second website (can't find it right now) where he listed all the products he would sell and it had a section on the top 5 pharmaceutical telomerase activators and the top 5 nutraceutical activators. Plus other sections for the most effective telomere protective substances. At some point the top nutraceutical result became licenced and I had assumed that this must be what is in Telo Vital. So, most likely only one of the telomerase activating blend shown in the attached ingredients is actually a powerful telomerase activator. But which one? 

[QuestforLife]

https://pubmed.ncbi....h.gov/15588713/ What do you think? Could prevent or cause arrythmias like in your case?

 

Does seem to suggest reduce arrythmias. I was using phloretin, which I later found blocked glucose uptake, so could have been a non-Rho kinase driven effect. 

[Advocatus Diaboli]

Re: post #1074

 

"So, most likely only one of the telomerase activating blend shown in the attached ingredients is actually a powerful telomerase activator. But which one?"

 

There is also the possibility that there is a synergistic effect among 2 or more of the ingredients resulting in powerful telomerase activation, correct?

[QuestforLife]

Possible, but I doubt it. I've asked Bill Andrews about Synergy studies before and I know he wants to do it, but don't think he has. Even if he has, surely a company buying exclusive rights wouldn't give away the combination they have licenced? I might fire an email to Dr Andrews and see what he says. 

[QuestforLife]

Possible, but I doubt it. I've asked Bill Andrews about Synergy studies before and I know he wants to do it, but don't think he has. Even if he has, surely a company buying exclusive rights wouldn't give away the combination they have licenced? I might fire an email to Dr Andrews and see what he says.

Okay so I was wrong. Bill says he discovered all the ingredients. According to this website, many activate telomerase. But they may be very specific extracts.

https://ultrarunner....duct/telo-vital

[Advocatus Diaboli]

QuestforLife,

 

In post #1074 you write:

 

"Kinda of suprising they just show the ingredients for all to see."

 

 

And, in post #1077 you write:

 

"...surely a company buying exclusive rights wouldn't give away the combination they have licenced?"

 

In the USA, for example, the FDA mandates that the ingredients in a supplement are listed on a label attached to the container of the supplement. Read the link for caveats.

 

Also, I suspect that it would be a waste of money to include ingredients that serve no function other than to, perhaps, "throw off" the competition. 

[Advocatus Diaboli]

Thanks for the link.