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DIY Rosmarinic Acid Eye Drops For Cataracts

rosmarinic acid cataracts eye drops

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#1 Daniel Cooper

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Posted 08 July 2019 - 05:55 PM


Based on this paper Rosmarinic Acid Restores Complete Transparency of Sonicated Human Cataract Ex Vivo and Delays Cataract Formation In Vivo and the absence of any commercial eye drop containing rosmarinic acid (RA), I'm toying with the idea of making my own DIY RA eye drops to address some early cataract formation.

This isn't something I take lightly. There's no clinical data whatsoever on topical RA for cataracts in humans, and I've very aware of the need to follow sterile procedures when making the preparation.

I'm thinking of making a 1% w/v solution. Clearly I'll need sterile distilled water and rosmarinic acid and a good scale. I suspect I'll also want to add a buffering agent to keep the pH in an acceptable range (say 6.0 - 8.0). Does anyone have any suggestions for a buffer agent? Clearly we don't want to neutralize the rosmarinic acid but we also don't want the pH going too low. I do have a digital pH meter and calibration solutions. 

 

Suggestions would be welcome.
 

 


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#2 Daniel Cooper

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Posted 08 July 2019 - 08:14 PM

Forgot to add.  RA is only slightly soluble in water. It is well soluble in most organic solvents and I believe it can be reasonably soluble in water if the right buffer agent is used.

 

I can't think of many organic solvents that I want to put in my eye, except maybe a very dilute solution of water + DMSO (which has actually been used at least experimentally in the eye in the past).  I suppose you might suspend it in olive oil or castor oil, however I would bet you'd get better permeability into the lens capsule with H20 + buffer or H20 + DMSO.

 

Again, comments from better chemists than I would be appreciated. 

 

 

 


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#3 Rays

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Posted 09 July 2019 - 09:49 AM

Interesting experiment.
Why not add some NMN?
Will you experiment on one eye only? To judge whether it has any effect?

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#4 Daniel Cooper

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Posted 09 July 2019 - 02:52 PM

I'm a little reluctant to just throw in things that "seem like a good idea" unless I have some evidence for effectiveness for cataracts.  

 

I could see that NR/NMN might be useful topically in the eye, but it would be nice to know that for sure.  I doubt it would hurt anything however.

 

And yes, I'd certainly start in one eye till I was comfortable with safety.  However, I think whether is effective will probably be obvious.  Either the cataract regresses or not.  They rarely (though not never) regress on their own.

 

 



#5 nickdino

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Posted 09 August 2019 - 09:08 AM

Interesting test man! Please keep us informed

#6 Oakman

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Posted 09 August 2019 - 04:21 PM

Interesting DIY. I used this as a base for some DIY eye drop experiment I did previously. Since it already has been shown (from reviews) harmless in the eye, I think it would be a good starting point. Good luck and keep us informed of your progress.


Edited by Oakman, 09 August 2019 - 04:22 PM.


#7 Rays

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Posted 06 September 2019 - 09:16 AM

How's it going, Daniel? Did you start your experiment?

 



#8 Rays

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Posted 06 September 2019 - 09:19 AM

Interesting DIY. I used this as a base for some DIY eye drop experiment I did previously. Since it already has been shown (from reviews) harmless in the eye, I think it would be a good starting point. Good luck and keep us informed of your progress.

 

The link didn't work; it went back to the same page. Can you post it again?



#9 Oakman

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Posted 06 September 2019 - 12:52 PM

The link didn't work; it went back to the same page. Can you post it again?

 

https://www.amazon.c...e?ie=UTF8&psc=1



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#10 Daniel Cooper

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Posted 06 September 2019 - 01:20 PM

My biggest issue right now is -

 

  • I'm having trouble sourcing rosmarinic acid.  Rosemary extract I can find, but it may have other constituents that I don't want to put in my eye.
  • I need a buffer to make the rosmarinic acid more soluble in water.  I'm thinking a phosphate buffer but my knowledge in this area needs some work.

  



#11 smithx

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Posted 07 September 2019 - 05:55 AM

Feeling helpful tonight. This is not an endorsement of the idea, since I haven't even read the paper yet:

 

 

Sources of rosmarinic acid:

https://www.caymanch...m/catalog/70900

https://www.sigmaald...ace=CAS No.&N=0

https://www.phytopur...acid-p-290.html

https://us.vwr.com/s...marinic-acid-97

http://www.kaimosi.com/n.html - Chinese - "free sample"

 

Solubility

Soluble in: water, 1425 mg/L @ 25 °C (est)

http://www.thegoodsc.../rw1372331.html

0.14% (ish)

 

Soluble in ethanol, DMSO or dimethyl formamide to approximately 25 mg/mL

https://pubchem.ncbi...tion=Color-Form
 

 

Rosmarinic  acid  is  supplied  as  a  crystalline  solid.  A  stock  solution  may  be  made  by  dissolving  the  rosmarinic acid in an organic solvent. Rosmarinic acid is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide. The solubility of rosmarinic acid in these solvents is approximately 25 mg/ml.Further  dilutions  of  the  stock  solution  into  aqueous  buffers  or  isotonic  saline  should  be  made  prior  to  performing biological experiments. Ensure that the residual amount of   organic solvent is   insignificant, since organic solvents may have physiological effects at low concentrations. Organic solvent-free aqueous solutions  of  rosmarinic  acid  can  be  prepared  by  directly  dissolving  the  crystalline  compound  in  aqueous  buffers. The solubility of rosmarinic acid in PBS (pH 7.2) is approximately 15 mg/ml. We do not recommend storing the aqueous solution for more than one day

https://www.caymanch.../pdfs/70900.pdf

 

 

 

PBS - Phosphate Buffered Saline - 7.2pH

https://www.thermofi...roduct/20012027

 

But:

 

Eye:May cause eye irritation.

http://www.uccaribe....ered-saline.pdf

 

So how about:

 

Sensitive Eyes Saline Solution

Boric Acid, Sodium Borate, Potassium Chloride, Sodium Chloride, Polyaminopropyl Biguanide, Edetate Disodium

https://www.walgreen...1684212-product

 


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#12 VivienGFFR

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Posted 19 January 2020 - 10:22 PM

https://www.coc.it/e...dose-containers

 

 



#13 YOLF

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Posted 20 January 2020 - 01:09 AM

MSM drops? Or did the Amazon seller change the listing to something else?


Edited by YOLF, 20 January 2020 - 01:11 AM.


#14 YOLF

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Posted 20 January 2020 - 01:18 AM

 

This eye drop looks interesting. From a contract manufacturer too. Sounds like a business opportunity.



#15 Oakman

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Posted 20 January 2020 - 03:01 AM

MSM drops? Or did the Amazon seller change the listing to something else?

 

No they haven't changed. read the 2nd bullet in the listing. Says eye drops, yes? Yes.



#16 Daniel Cooper

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Posted 22 January 2020 - 06:10 PM

 

I would love to get ahold of some of that. But can't justify starting up a business to try to import it.



#17 VivienGFFR

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Posted 22 January 2020 - 07:15 PM

I emailed them to ask for a sample but nothing. Maybe I will try LEF Brite Eye III for my corticotherapy induced cataract and give it a try before regular surgery as this would be a bit tricky in my case.



#18 Daniel Cooper

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Posted 22 January 2020 - 07:22 PM

I emailed them to ask for a sample but nothing. Maybe I will try LEF Brite Eye III for my corticotherapy induced cataract and give it a try before regular surgery as this would be a bit tricky in my case.

 

 

I tried Brite Eye III for three months and nothing.  The problem is that all the research showing a positive effect on cataracts from those N-Acetylcarnosine drops was done by the same Russian group that holds the patent.  I've seen no independent research that shows it is effective so far.



#19 VivienGFFR

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Posted 22 January 2020 - 07:40 PM

I tried Brite Eye III for three months and nothing.  The problem is that all the research showing a positive effect on cataracts from those N-Acetylcarnosine drops was done by the same Russian group that holds the patent.  I've seen no independent research that shows it is effective so far.

 

Ok thanks for sharing your experience. So you would preferably recommand a rosmarinic acid product ?



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#20 Daniel Cooper

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Posted 22 January 2020 - 08:21 PM

Ok thanks for sharing your experience. So you would preferably recommand a rosmarinic acid product ?

 

 

Oh heaven no.  The only thing I know is that rosmarinic acid solutions cleared cloudy lens in vitro. I haven't seen an animal or human study.  I don't think anyone's done one yet. I just think I can make a relatively weak rosmarinic acid solution that is very unlikely to be harmful that might help with cataracts.  

 

At this point the only opinion I have is on what doesn't work. From my experience, N-Acetylcarnosine drops don't work (or at least didn't for me) nor did Lanosterol eye drops work.  

 

Rosmarinic acid shows one of the strongest cataract reversal effects in vitro. Whether or not that would translate into an in vivo effect is anyone's guess.  But, when you're trying to reverse or at least delay cataracts, there aren't many games in town.  As long I think it won't hurt me, I'm willing to give it a try.  But what I'm willing to try should have no influence on you.  I'm not a doctor after all.

 

The only other thing out there that is under clinical study is EV-06, aka dioptin, aka UNR844, aka lipoic acid choline ester.  It's being developed by Novartis for presbyopia.  It works by breaking disulfide bonds which cause the lens to stiffen as we age so that we lose focal accommodation.  It is believed that the formation of these same disulfide bonds also leads to cataracts, so in theory this compound may work for this as well.

 

It's in FDA trials at the moment and I haven't figured out exactly where it is in the process.  Maybe it will pop out as an approved drug you can buy in a few years.  Sigh.


Edited by Daniel Cooper, 22 January 2020 - 08:22 PM.






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