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Stem cell self-renewal with C60

c60 stem cells mitochondria fusion stearic acid aging hydroxytyrosol olive oil mct oil proliferation

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#1051 lost69

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Posted 10 November 2019 - 11:05 AM

I threw away all the c60oo I had in the freezer.  I had added way too much hydroxytyrosol, and experienced no long-term benefits (albeit spurts of rejuvenation).  Wary of c60oo, I have stayed on the sidelines of this protocol, checking back now and again to see how its coming along. 

 

Now I'm looking at giving the protocol a go. Part of my thinking is it could help to compensate for damage caused by c60oo (replenish stem cells).  Moreover, the additional exposure to c60 in the protocol looks small compared to what I consumed in the past.  Also, as I have done a round of high-dose fisetin, and can see doing more rounds of it, ignoring the stem cell situation might not be smart. 

 

Is it safe to use the ses c60 that I bought 4 or 5 years ago?  It's been stored in a box in a closet in an air-conditioned room.  If I should toss the old stuff, what is the best option today for obtaining c60 material of the highest quality?  

 

As I am sensitive to polyphenols in olive oil, what would be the best oil to substitute?  (If  substituting olive oil will negate the benefits, then at least it won't be adding the extra HT which is what had caused me the most trouble in the past). 

 

i was also wondering if c60 from SES, ordered 1-2 years, ago is still good.i immediately put the box in freezer at -20°
 



#1052 aribadabar

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Posted 18 November 2019 - 04:13 AM

i was also wondering if c60 from SES, ordered 1-2 years, ago is still good.i immediately put the box in freezer at -20°
 

 Yes, it would be fine. (GVA is a C60 researcher)



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#1053 dlewis1453

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Posted 06 December 2019 - 07:25 PM

Hi Turnbuckle, 

 

Could you elaborate a bit on what you perceive to be the main risks of using this protocol? 

 

For example, I have seen you allude to unknowns regarding the consumption of C60, as well as the risk of starting this protocol if you are still fairly young (perhaps because of long term impacts on the stem cells?). 



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#1054 Turnbuckle

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Posted 06 December 2019 - 08:18 PM

Hi Turnbuckle, 

 

Could you elaborate a bit on what you perceive to be the main risks of using this protocol? 

 

For example, I have seen you allude to unknowns regarding the consumption of C60, as well as the risk of starting this protocol if you are still fairly young (perhaps because of long term impacts on the stem cells?). 

 

My experience with C60 goes back to early 2012. The stem cell effects were clear, but faded fairly rapidly, suggestive of stem cell depletion. I thus looked for a way of preventing that depletion, and found it with mito fusion, which I had previously used with mito fission to clear out defective mitochondria. C60 and mito fusion provide the two switches that force stem cell self renewal. The results are impressive, but my experience with it amounts to less than 2 years at present. I've said this is a geriatric protocol because the risks are minimal with the old. The largest unknown is the number of times stem cells can be multiplied. Certainly if you are running out, it makes sense to multiply them, but if you are young and have healthy stem cell pools, multiplying them is wasteful. There appear to be homeostatic mechanisms that maintain stem cell pools at a certain size. If you expand them past that, the body gets rid of the excess without any benefit. The mechanisms are hazy (and varied, given the variety of SC niches), but appear to employ signaling molecules that direct SC's to symmetric differentiation. 

 

...the maintenance of a constant pool of stem cells (SCs) can be accomplished by one of two distinct types of cell division during tissue homeostasis (see the figure): in an asymmetric division, one daughter remains a SC throughout self-renewal, and the other daughter becomes committed to enter a programme of terminal differentiation. This asymmetrical cell fate specification can be achieved either by the unequal segregation of a cell fate determinant, either proteins or RNAs (red line), or by positioning one of the daughters away from the SC niche27,28,102. By contrast, symmetrical cell divisions result in both daughters adopting the same fate, which for SCs would result in the generation of two SCs (a process that is called symmetric self-renewal) or two differentiated cells (a process that is called symmetric differentiation).

https://www.ncbi.nlm...les/PMC2760218/

 


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#1055 dlewis1453

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Posted 06 December 2019 - 11:20 PM

"The results are impressive, but my experience with it amounts to less than 2 years at present. I've said this is a geriatric protocol because the risks are minimal with the old. The largest unknown is the number of times stem cells can be multiplied." 

 

"There appear to be homeostatic mechanisms that maintain stem cell pools at a certain size. If you expand them past that, the body gets rid of the excess without any benefit." 

 

Regarding your language above that I emphasized in bold- is your concern that a young person multiplying stem cells in this manner frequently over a long period of time could age themselves prematurely?

 

Regarding the size of the stem cell pools, I imagine that the size of the various pools varies throughout the body. So pool A may be filled up after just 4 rounds of this protocol, but pool B may require 12 rounds. Therefore some wasteful divisions in pool A will be necessary to complete the process of filling up pool B. This may be further complicated by differing penetration rates of C60 throughout different tissues in the body. Interestingly, your benefits appear to be body wide, including in places as disparate as the skin, eyes and brain, so that suggests that thankfully your protocol is reaching many of the stem cell pools .  

 

How is your blood pressure lately? I seem to remember it was improving at one point. If that trend continued, that could indicate renewal of stem cells in the endothelium. 


Edited by dlewis1453, 06 December 2019 - 11:30 PM.


#1056 Turnbuckle

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Posted 07 December 2019 - 04:06 PM

Regarding your language above that I emphasized in bold- is your concern that a young person multiplying stem cells in this manner frequently over a long period of time could age themselves prematurely?

 

It seems possible. But using C60 without fusion would be even worse, and many people are doing it.

 

Regarding the size of the stem cell pools, I imagine that the size of the various pools varies throughout the body. So pool A may be filled up after just 4 rounds of this protocol, but pool B may require 12 rounds. Therefore some wasteful divisions in pool A will be necessary to complete the process of filling up pool B. This may be further complicated by differing penetration rates of C60 throughout different tissues in the body. Interestingly, your benefits appear to be body wide, including in places as disparate as the skin, eyes and brain, so that suggests that thankfully your protocol is reaching many of the stem cell pools .  

 

C60 has been shown to penetrate all tissues measured. Fusion in the brain, however will require sulforaphane, as stearic acid is blocked by the BBB.

 

How is your blood pressure lately? I seem to remember it was improving at one point. If that trend continued, that could indicate renewal of stem cells in the endothelium. 

 

My BP was never improved by SC treatment. In fact, mito fusion tends to raise BP.

 



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#1057 Empiricus

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Posted 07 December 2019 - 09:21 PM

Regarding your language above that I emphasized in bold- is your concern that a young person multiplying stem cells in this manner frequently over a long period of time could age themselves prematurely?

 

 

It seems possible. But using C60 without fusion would be even worse, and many people are doing it.

 

Quite a number of non-elderly used c60 without fusion in the past.  Some took a little, some moderate amounts, some used a lot.  Are they all better to hold off until they are geriatrics, or could this protocol help repair the damage (compensate for any over-depletion)?   



#1058 dlewis1453

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Posted 09 December 2019 - 03:11 PM

Quite a number of non-elderly used c60 without fusion in the past.  Some took a little, some moderate amounts, some used a lot.  Are they all better to hold off until they are geriatrics, or could this protocol help repair the damage (compensate for any over-depletion)?   

 

This protocol could help reverse the depletion. Turnbuckle says he used c60 without fusion extensively for a while and appeared to begin developing symptoms of stem cell depletion, but this protocol reversed that depletion and made him younger. 


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#1059 Graviton

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Posted 10 December 2019 - 01:11 AM

Any one uses cocoa butter(or high cocoa butter chocolate) instead of stearic acid? Can palmitic acid in cocoa butter impact on mitochondrial fusion effect of stearic acid in cocoa butter?


Edited by Graviton, 10 December 2019 - 01:12 AM.


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#1060 Puppalupacus

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Posted 10 December 2019 - 01:52 AM

Frankly, I found all of this to be utterly useless.  Nice science and logic, but in the end, completely ineffective.  Growing feet?  Come on.


Edited by Puppalupacus, 10 December 2019 - 01:52 AM.

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#1061 dlewis1453

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Posted 10 December 2019 - 04:24 AM

Frankly, I found all of this to be utterly useless. Nice science and logic, but in the end, completely ineffective. Growing feet? Come on.


Can you elaborate? Did you attempt the protocol and notice no benefit?
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#1062 QuestforLife

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Posted 10 December 2019 - 09:49 AM

Frankly, I found all of this to be utterly useless.  Nice science and logic, but in the end, completely ineffective.  Growing feet?  Come on.

 

I've done cycles of this protocol plenty of times in the past and not had any noticeable results from it. I'm 41. It would be interesting to get a survey done to see what is causing the variable results, i.e. age, C60 source, stearic acid used, other supplements taken with it, etc. 



#1063 Turnbuckle

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Posted 10 December 2019 - 10:48 AM

I've done cycles of this protocol plenty of times in the past and not had any noticeable results from it. I'm 41. It would be interesting to get a survey done to see what is causing the variable results, i.e. age, C60 source, stearic acid used, other supplements taken with it, etc. 

 

I don't advise anyone under geriatric age to use this protocol. If you still have sufficient stem cells and few senescent cells, you won't achieve much of anything except to create stem cells that are removed and thus wasted by homeostatic mechanisms.


Edited by Turnbuckle, 10 December 2019 - 10:53 AM.

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#1064 dlewis1453

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Posted 10 December 2019 - 03:18 PM

I've done cycles of this protocol plenty of times in the past and not had any noticeable results from it. I'm 41. It would be interesting to get a survey done to see what is causing the variable results, i.e. age, C60 source, stearic acid used, other supplements taken with it, etc. 

 

 

Hi QuestforLife, 

 

Have you ever tried C60 alone and received  any rejuvenation benefit from it? 



#1065 QuestforLife

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Posted 10 December 2019 - 04:17 PM

Hi QuestforLife, 

 

Have you ever tried C60 alone and received  any rejuvenation benefit from it? 

 

Yes some good results in 2013-2014, but they were unreliable - high energy for a while, then low energy, short lived mental clarity, alcohol resistance if used over a few days before consumption. I haven't noticed any benefits using it intermittently with the protocol.

 

I've bought from the following: Vaughter Wellness, Carbon60 and Carbon60 Plus (not actually olive Oil from the Livepet people). Vaughter Wellness is what I've used the most as I'm in the UK and I got good if inconsistent results (see above) from it. But it tastes different now and I'm unsure if they've changed the olive oil or it has become oxidised.

 

I've used actual Stearic acid from sigma, dissolved in dark chocolate to make a nice drink. But I'm skeptical you need it as pure as I've used - you get more bioavailabilty mixed up in a dark chocolate say; pity they haven't done a study using actual foodstuffs. 

 

So who here has had positive results from Turnbuckle's protocol? 

 

Maybe when you are much older your organ and blood stem cell pools are so low you can easily notice a boost when they are increased in size. I've noticed much more results by stimulating a release from the bone marrow using AFA or similar - but I always worry about depletion (more youthful now, but shorter life). 



#1066 Kentavr

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Posted 10 December 2019 - 04:31 PM

Yes some good results in 2013-2014, but they were unreliable - high energy for a while, then low energy, short lived mental clarity, alcohol resistance if used over a few days before consumption. I haven't noticed any benefits using it intermittently with the protocol.

I've bought from the following: Vaughter Wellness, Carbon60 and Carbon60 Plus (not actually olive Oil from the Livepet people). Vaughter Wellness is what I've used the most as I'm in the UK and I got good if inconsistent results (see above) from it. But it tastes different now and I'm unsure if they've changed the olive oil or it has become oxidised.

I've used actual Stearic acid from sigma, dissolved in dark chocolate to make a nice drink. But I'm skeptical you need it as pure as I've used - you get more bioavailabilty mixed up in a dark chocolate say; pity they haven't done a study using actual foodstuffs.

So who here has had positive results from Turnbuckle's protocol?

Maybe when you are much older your organ and blood stem cell pools are so low you can easily notice a boost when they are increased in size. I've noticed much more results by stimulating a release from the bone marrow using AFA or similar - but I always worry about depletion (more youthful now, but shorter life).


The TRIIM protocol haunts me: it really is a real find!

It just needs to work well in middle age.

Edited by Kentavr, 10 December 2019 - 04:32 PM.


#1067 genX

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Posted 10 December 2019 - 05:39 PM

As far as I know, no one here has commented on the use of stem-cell treatment to boost stem-cells etc.   There is a local doctor in my town who seems to having a lot of success for a variety of conditions by using adipose stem cells (derived from belly fat) as well as exosomes (which I think comes from a Florida company which obtains them from discarded placentas). 

 

What about using injections of adipose stem-cells to increase available stem cells and/or exosomes to "turn them on"?

 

P.S. I've tried C60 olive oil from a few brands for short times and small doses to see if it could help reduce the ringing in my right ear, and the only effect I found was that it made it worse.  Although, it's there may have been some after-effect benefits after I stopped taking it. 

 

P.P.S. The mechanisms of stem-cell treatment effects have not been discussed either, but from what I've read it appears it is more due to cytokines that they release (which stimulate healing in various other cells of the body) rather than what had been thought in the past, e.g. that they "go to the affected area and replace/multiply/fix damage"

 

 



#1068 Turnbuckle

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Posted 10 December 2019 - 06:21 PM

Yes some good results in 2013-2014, but they were unreliable - high energy for a while, then low energy, short lived mental clarity, alcohol resistance if used over a few days before consumption. I haven't noticed any benefits using it intermittently with the protocol.

 

Expect the high energy comes from UCP2 blocking of somatic cells and the alcohol resistance from its antioxidant properties. Both are temporary effects.

 

I've used actual Stearic acid from sigma, dissolved in dark chocolate to make a nice drink. But I'm skeptical you need it as pure as I've used - you get more bioavailabilty mixed up in a dark chocolate say; pity they haven't done a study using actual foodstuffs. 

 

It's possible that stearic acid will react with certain ingredients and thus become less available. Sodium stearate, for instance, has very low bioavailability. The glycerol in triglycerides locks it up and prevents undesirable reactions -- as long as it isn't tristearin, which is also not very available. In any case, I expect that your failure to get perceptible results at 41 comes from your not being aged. It's like taking a drug for a disease you don't have. You won't get much from penicillin if you don't have an infection.


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#1069 dlewis1453

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Posted 11 December 2019 - 07:15 PM

The article attached below discusses a procedure in which stem cells were transferred to women's ovaries to reverse premature menopause.

 

Turnbuckle and others - are we aware of any women who have used this protocol? I wonder if they have had any interesting results. 

 

"In the two participants who have completed the treatment to date, serum estrogen levels have increased as soon as 3 months after the injection of stem cells, and the effect has lasted for at least one year. Their menopausal symptoms have been alleviated, and six months after the injection of the stem cells into the ovaries, they have resumed menses,"

https://www.scienced...80318144839.htm



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#1070 dlewis1453

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Posted 11 December 2019 - 07:55 PM

 

 

Turnbuckle and others - are we aware of any women who have used this protocol? I wonder if they have had any interesting results. 

 

 

 

Sorry, I should have been more clear. Are we aware of any women who have used the C60 stem cell renewal protocol? 

 

I suspect the C60 protocol could have results similar to the procedure discussed in the article. 







Also tagged with one or more of these keywords: c60, stem cells, mitochondria, fusion, stearic acid, aging, hydroxytyrosol, olive oil, mct oil, proliferation

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