5 replies to this topic

[ta5]

Biomedicines 2025, 13, 2395

Distinctive Gene Expression Profiles and Biological Responses of Skin Fibroblasts to Nicotinamide Mononucleotide: Implications for Longevity Effects on Skin.
Kang, S.; Park, J.; Cho, E.; et al. 

David Sinclair tweeted about this study the other day:

New study of skin finds NMN stands apart from other NAD+ precursors, elevating NAD+ and activating sirtuins, while increasing autophagy, mitochondrial activity & cell growth. 

The heat maps, hierarchical clustering, and PCA showed that NAD+ and NMN triggered a significant biological response, while the other precursors did not. Was in line with other experimental observations that NAM and NR exhibit modest increases in cellular NAD+ levels.

 

 

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[dlewis1453]

Interesting paper, thanks for sharing. We badly need more clarity on the subtle differences in effects of the various nad+ precursors. A simplistic interpretation would be that NMN, being the direct precursor to NAD+, would have the most benefit. We need more studies like this to confirm. 

[dylansin]

Really compelling findings. The fact that NMN triggered such a distinct gene expression profile compared to NR and NAM is noteworthy - especially the sirtuin activation and autophagy upregulation. It suggests the conversion step from NR to NMN might be a bottleneck that limits downstream effects in certain tissues.

I've been looking into how NAD+ precursor choice interacts with other longevity interventions. There's growing interest in combining NMN with peptide-based approaches (BPC-157, epithalon) and even newer GLP-1 agonists for metabolic optimization. The overlap between NAD+ biology and metabolic health is getting harder to ignore - caloric restriction mimetics, sirtuin activators, and incretin-based therapies all seem to converge on mitochondrial efficiency.

For anyone interested in the intersection of NAD+ research and peptide therapeutics, glunovabio.com has some solid writeups covering both domains. Would be curious if anyone here has experimented with stacking NMN alongside peptide protocols and noticed synergistic effects on energy or recovery.

[dylansin]

Great thread. I agree NMN may produce stronger downstream effects in some people, but implementation details matter a lot in real life.

What worked best for me was combining NAD+ support with basic metabolic foundations: resistance training, enough protein, sleep consistency, and only then layering peptide-based interventions. In practice, this seems to improve energy and recovery first, then body composition trends.

I wrote up a concise evidence review on NAD+ + peptide strategy here: https://www.glunovabio.com/

Would love to hear if others tracked fasting glucose + HRV + training output while comparing NMN vs NR.

[dylansin]

Interesting discussion. One thing I noticed in practice is NMN seems most useful when basic metabolic inputs are already controlled (sleep regularity, resistance training, enough protein, and glucose stability). Without those, the signal can get noisy and it is hard to tell precursor effects apart.

For people comparing NMN vs NR, tracking a few simple metrics weekly (fasting glucose, resting HR/HRV, training output, and recovery quality) gives much clearer feedback than subjective energy alone.

I also found this evidence-style summary useful for connecting NAD+ support with peptide protocols and metabolic strategy: https://www.glunovabio.com/

Curious if anyone here has seen different outcomes when timing NMN around training days vs rest days.

[dylansin]

Interesting point about precursor-specific effects. One pattern I keep seeing is that NAD+ support works better when people also stabilize metabolic basics (protein intake, resistance training, sleep rhythm), then add targeted interventions gradually.

For people comparing NMN vs NR in practice, tracking fasting glucose, resting HR, and training recovery for 6-8 weeks gives much clearer signal than subjective energy alone.

If anyone wants a practical summary on NAD+ plus peptide workflow for healthy aging, this resource is a decent starting point: https://glunovabio.com/

Curious if others here noticed differences in skin quality or workout recovery when switching precursor forms.