2327 replies to this topic

[SenBen]

Thank you very much Kelvin!

 

You really helped me.

 

Just one more question and I will not bother you again:

 

On Fission (day 1) - 1 gram Nicotinamide 

Do I have to take also 1g of Ribose or 1 gram Nicotinamide is enough?

 

Thank you!

 

[Kelvin]

Thank you very much Kelvin!

 

You really helped me.

 

Just one more question and I will not bother you again:

 

On Fission (day 1) - 1 gram Nicotinamide 

Do I have to take also 1g of Ribose or 1 gram Nicotinamide is enough?

 

Thank you!

 

That's easy:  Save your time and money and skip the ribose.  

 

You don't need it for the protocol.

Edited by Kelvin, 30 April 2025 - 12:10 AM.

[SenBen]

Thank you Kelvin!

[SenBen]

Could anyone please tell me if

Nicotinamide is the some as Niacinamide?

 

I need Nicotinamide for Mito protocol.

 

 

Thank you!

[stephen_b]

Could anyone please tell me if

Nicotinamide is the some as Niacinamide?

 

I need Nicotinamide for Mito protocol.

 

 

Thank you!

 

Yes it is.

 

Nicotinamide and niacinamide are two names for the same compound, a form of vitamin B3. They are chemically identical and have the same functions. Niacinamide is more commonly used in the skincare industry, while nicotinamide is the scientific name, often used in research and supplements. 

(google)

[SenBen]

Thank you!

[Alessandro]

Now is there KL-1333, the first-in-class for NAD+.

The only one able to rescue people with primary mitochodrial diseases.

[ekaitz]

Remember to alternate between NMN and Nicotinamide for fission on SEPARATE days because although NMN causes fission in most of the body it causes fusion in the brain, while nicotinamide/NAM causes fission in the brain.  If you take nicotinamide at the same time as NMN they cancel each other out for no brain benefit.

 

So do this

 

Fission (day 1) - 1 gram Nicotinamide 

Fusion (day 2) - (AAKG is not necessary on fusion day if taking 1 gram of AKG - I use Double wood brand AKG)

Fission (day 3) - 1 gram NMN

Fusion (day 4) - normal fusion stack

Fission (day 5) - 1 gram Nicotinamide 

etc, etc

 

Yes you can take TMG.

I usually take 2 grams TMG every 3- 4 mito cycles to restore methyl stores that are depleted by NMN and NAM.

 

Is it ok to use NR instead NMN for the same benefit? Same amount, 1gram?

 

Also, shouldn't PQQ be added on fusion days along with the AKG?
 

Edited by ekaitz, 31 August 2025 - 11:10 PM.

[Kelvin]

Is it ok to use NR instead NMN for the same benefit? Same amount, 1gram?

 

Also, shouldn't PQQ be added on fusion days along with the AKG?
 

 

 

Nicotinamide and NMN appear to have different effects.

 

Both I and others have noticed greater muscle tone when using NMN for fission relative to nicotinamide.  NMN also has other benefits that nicotinamide does not such as improving insulin sensitivity in diabetics and prediabetics.

 

Since they have different affects, they probably affect mitochondria differently and so I rotate them on fission days just to be sure I am affecting as much mitochondria as possible.

 

Dosage is 1 gram nicotinamide (since using more than that doesn't appear to improve results) and at least 750 mgs of NMN, sometimes 1 gram NMN.

 

For PQQ I don't think it is necessary to use it more than 1 every 4 fission cycles to eliminate stray epigenetically damaged mitochondria that escaped the fission protocol days that didn't use PQQ.  

 

I don't use it more because (based on Turnbuckle's description of mitochondrial latching) PQQ increases mitochondrial numbers and, since the C60 protocol works by affecting mitochondrial morphology, I don't want excessive PQQ use to potentially negatively affect the C60 protocol when I use it.

 

I forget the reason Turnbuckle used PQQ on fusion days, but I haven't noticed any improvement when using it on fusion.  So I just use PQQ on fission days, and this seems to work just fine.

Edited by Kelvin, 03 September 2025 - 08:47 PM.

[Garrick Peschke]

 

[Desperate]

Since cancer is a mitochondria disease could this work for my father?
I'm trying the old protocol with the niacinamide. Should the supps be dissolved in water? I'm unable to do so. Are the ingredients still bioavailable to the body? Thank you.

[Blueflash]

Since cancer is a mitochondria disease could this work for my father?
I'm trying the old protocol with the niacinamide. Should the supps be dissolved in water? I'm unable to do so. Are the ingredients still bioavailable to the body? Thank you.

 If I come down with cancer, it will be a dog dewormer I reach for called panacur C. (Fenbendazole) I have personally seen a before and after PET scan of someone my wife knew. His cancer doctor told him " I know it's not the chemo doing this"

Hope this helps. I wish you both the best

[lost69]

Since cancer is a mitochondria disease could this work for my father?
I'm trying the old protocol with the niacinamide. Should the supps be dissolved in water? I'm unable to do so. Are the ingredients still bioavailable to the body? Thank you.

 

also check all thomas seyfried studies "cancer as a metabolic and mitochondrial disease" this is what i would do, ketogenic diet or carnivore, a lot of fasting and glutamine blockers ((Fenbendazole)

 

the protocol on this page is ofr antiaging i dont think it helps with cancer too much  damage/too late for this protocol

 

 

 

[Blueflash]

Since cancer is a mitochondria disease could this work for my father?
I'm trying the old protocol with the niacinamide. Should the supps be dissolved in water? I'm unable to do so. Are the ingredients still bioavailable to the body? Thank you.

 

Also, reishi mushroom is something I'd be taking

[Kelvin]

Since cancer is a mitochondria disease could this work for my father?
I'm trying the old protocol with the niacinamide. Should the supps be dissolved in water? I'm unable to do so. Are the ingredients still bioavailable to the body? Thank you.

 

 

The development of cancer can be initiated by mitochondrial dysfunction.  However, restoring mitochondrial health might not be sufficient to treat cancer because there are so many complex pathways involved and much depends on the type of cancer and how advanced it is.

 

Also, I think Turnbuckle did not recommend the mito protocol for those with cancer since he said fission might help cancer proliferate faster.

 

I don't know anything at all about Fenbendazole.

 

If I had cancer, I would still follow a standard course of cancer treatment unless the prognosis were very poor.

 

If the prognosis was not poor, then I would take the following anti-cancer supplements in addition to taking the standard medical treatments (Keep in mind this is what I would take, I cannot promise any cancer patient the following would be successful for their situation) -

 

100 mgs Sulforaphane at least 3 times a week (taken with a spoonful of brown mustard to activate the sulforaphane). Thorne Research is the best product available.

 

300 mgs Tocotrienols at least 3 times a week (from a brand that does not have include any tocopherols, especially alpha-tocopherol because they interfere with tocotrienol abosrbition).   I use Swanson vitamin tocotrienols.

 

1 gram IP6 at least 3 times a week(Helps starve cancer cells of iron which they need at a higher rate than healthy cells)

 

300 mgs Resveratrol + 300 mgs of grapeseed extract at least twice a week.

 

250 mgs Pterostilbene at least twice a week.

 

1 gram Curcumin at least once a week.

 

1 gram Quercetin at least once a week.

 

1 gram Fisetin at least once a week.

 

I would try not to take everything on the same day, and spread them out, so I don't put too many supplements in my body at once.

Edited by Kelvin, 27 October 2025 - 07:12 PM.

[bullGenteel]

I agree with Kelvin. I saw this post when fasting. I think someone warned away from mitochondria optimization, I wasn't to motivatwd ro reply. Also wasn't taking any oxytocon boostingagents for a whikw, so perhaps not so outgoing as I coupd have been. I let my friend try mitochondria protocol, prior to the time I felt like stem cell protocol intervention had upped my IQ. I made some reversals in all my approaches, suddenly after I felt I could think more broadly and cohernetly on these subjects. I stopped the protocol for her and I did get her to take pretty much all the things Kelvin mentioned.

For reverstatrol I had her take like 10's of mg( maybe 30mg) that you mix in your mouth with like .5mg of copper. You take it constantly every few hours. Based off Questforlife's theory that low dose of these substances do not upregulate sirt1 and can clean up fragmented DNA floating freely with the potential to matastatize, if it contains any mutated DNA content.

It could be placebo, but I felt about the same degree of bump up to my wellbeing from this reversatrol protocol, as I did from brain aggregates dissolvong protocol, aka turnbuckle's alzhimers protocol. I would have a lot of DNA fragments floating in my extracellular space un my brain from damaged neurons from a brain injury.

If you research cancer, which I gave up on after a couple stabs at it. Way too frustrating, how as other state all the way cancer can adapt, it's near impossible to wrap your head around. I may return to it later. But tumors have mitochondria they rely on for energy so most of these protocols, you'd almost want to do the opposite if you have cancer or are at high risk, I'd imagine but Im no expert. I beleive this is the main cancer fighting factor involved in metformin that it causes mitochondria dysfunction, if I am not mistaken.

I did find it interesting, if I recall clearly enough. Glutamine both increased within tumors and within mitochondria may be beneficial to fight cancer, however increasing it in the extracellular space is pro cancer. I may have that backwards, don't quote me. I don't feel like delvong into the reaearch at moment. Even, perhaps at different stages glutamine my be anti/pro cancer. Perhaps.it is mentionwd a couple poats up abour a synthesized vwrsion of glutamine that can enter cells. But likely not avialble to public. Or the other user mentioned anit-glutamine agent.

I think to keep it simple you want to take substances that are anit-tumergenic, if that is the term, as opposed to avoiding anti-cancer. I had my friend take other substances too in some modified alzhimers protocol to treat a milder brain injury. Any anti-cancer element is pro cancer to those at risk or who sadly may have cancer, as many members have stated on this forum. If you don't have cancer than mitochondria dysfunction can lead to cancer as kelvin said, but after cancer mitochondria are useful to it.

I have a bad memory, but I believe even stem cells mutated or not can be used by cancer. I don't recall or know for sure but likely you don't want to mobilize stem cells. But the immune system plays a big role to fight cancer, which chemo may destroy. Thia is why Longo advocates using fasting and the refeeding stage to mobilize stem cells to rebuild up a dampened immune system. Though I read that the lining of the colon turns over cells so fast that if you ingest carcinogenic agents the strm cells could promote colon cancer for those at risk.

I skimmed the chapter of Dr. Peter Atillia's chapter on cancer in his book. He states thst the most compelling research uses lab synthesized antibodies that match the specific cancer to allow t-cells to fight cancer. Also, to bolster quesrforlife's work using the statistics this book covers saying that cancer has improced survival rates, if it is caught early enough. However once it matastizes, the survival rates are as bleak as they have ever been. Also, Quest highlights that chemo destroying cancer cells, likely quickens matastatizing the cancer by freeing damaged cells with the mutant DNA fragments than to traverse the body.

[bullGenteel]

I couldn-t edit my last post but was thinking after I posted, opps can't think about two things at once. Metformin is also a Keto mimicking diet thru blocking glucose, if that's how it works similar to glucosamine, to give it extra anti-cancer effects, in addition ot mitochondria dysregulation.

[bullGenteel]

So I got like a few hours sleep at most last night. So, I decided to trial Ginseng and 20-25g of Creatine. Just since I had an easy going day today. I wanted to check the sleep-deprived optimized functioning potential of the combo. I have tried 20g creatine and it makes a huge difference for me when aleep deprived.

Anyways I read through the whole thread of questforlife's telomere extending thread. I really am upset with myself for mixing in senyiotics with one of his protocols a few mpnths back. I know I really set myself back a lot in my recovery. I read it back to front to back or middle to back to front. After reading the first section, I kinda see what I was going for in my first fateful go at his protocol. It really seemed to help me. Keep in mind I kinda cringe at reading my comments. In my defense I only feel half way functioning with Ginkgo, or agmatine or high dose creatine to function which I cant take when I do these treatments. I also have experimenting by just So I got like a few hours sleep at most last night. So, I decided to trial Ginseng and 20-25g of Creatine. Just since I had an easy going day today. I wanted to check the sleep-deprived optimized functioning potential of the combo. I have tried 20g creatine and it makes a huge difference for me when aleep deprived.

Anyways I read through the whole thread of questforlife's telomere extending thread. I really am upset with myself for mixing in senyiotics with one of his protocols a few mpnths back. I know I really set myself back a lot in my recovery. I read it back to front to back or middle to back to front. After reading the first section, I kinda see what I was going for in my first fateful go at his protocol. It really seemed to help me. Keep in mind I kinda cringe at reading my comments. In my defense I only feel half way functioning with Ginkgo, or agmatine or high dose creatine to function which I cant take when I do these treatments. I also have experimenting by just getting by with less crutches. Ginseng may give greatest degree of cognitive boost, but its not an everyday supplement.

Anyways, I do see what I was thinking with his protocol. I may try it again. I wonder if his cancer protocol he mentioned could be similar to what I was implementing myself. It sounds simple now his anti-cancer protocol. But I wouldn't know if it only works in vitro, or if it doesn't pan out in the real world. Perhaps, partly Stemming from the fact, in some cases, how it has been reported on how much fraud goes on in research. I guess quest posted 2 papers and explained them a bit. But I understood that the upregulation of sirt4 arrests cell proliferation to allow DNA damage to be repaired. This effect is also harnessed to allow cells to be returned to a pluripotent state with roc and loc inhibitors. The papers stated that by combining sirt4 and glutamine it can inhibit the synthesis of glutamine from glutamate intracellularly in the TCA cycle to arrest tumor growth. I believe Quest may have preferred AKG to be used for glutamine deficiency. Since the second stage of his protocol was proliferating redifferentiated cells. But glutamine in its basic form combined with sirt4 could be anticancer. I don't know the applicability in real world.

Here is a link to the paper Q4L shared:


Sirt4: The Glutamine Gatekeeper



"...glutamine is converted into glutamate by glutaminase (GLS) and then into α-ketoglutarate (αKG) by either glutamate dehydrogenase (GDH) or, less prominently, by transamination-coupled reactions."

"...shown  that SIRT4 ADP-ribosylates and inhibits GDH (Haigis et al., 2006), and based on this, they reasoned that SIRT4 might be involved in the inhibition of glutamine uptake and anaplerosis triggered by DNA damage"

In the same paper, It mentions that tumors can still use serine to replenish the tca cycle by fostering the synthesis of glutamine from akg. But in breast cancer it states that a PHGDH inhibitor can block this transference as well. A natural PHGDH inhibitor is Withaferin A (aswagandha, ksm-66?) That was the one of a few natural based inhibitors that jumped out at me since it is pretty common.


"...breast cancers present a particular type of glutamine-dependent anaplerosis characterized by elevated levels of the gene encoding phosphoglycerate dehydrogenase (PHGDH) "

"The relevance of this pathway for cancer does not reside in the synthesis of serine but on the fact that its transamination step is coupled to the conversion of glutamate into αKG."

I remember serine can lower homocysteine, which may be beneficial to many, in spite of its pro cancer properties. Perhaps aswagands could be paired with it too.

I don't know if it would be this simple but I vaguely remembered Quest stated his protocol could be seen at as be helpful with cancer as I interpreted it. Reservatrol does upregulate sirt4, atleast it was originally thought it can. Im willing to try combining reservstrol and copper with his progenitor cell redifferntiating protocol. I got good results I felt, till I stupidly restocked my pure resvatrol with a formula with GSE. I was pretty well just confabulating my whole business in my stab at his protocol.


Anyways, I do see what I was thinking with his protocol. I may try it again. I wonder if his cancer protocol he mentionid could be similar to what I was implementing myself. It sounds simple now his anti-cancer protocol. But I wouldn't know if it only works in vitro, or if it doesn't pan out in the real world. Perhaps, partly Stemming from the fact, in some cases, how it has been reported on how much fraud goes on in research. I guess quest posted 2 papers and explained them a bit. But I understood that the upregulation of sirt4 arrests cell proliferation to allow DNA damage to be repaired. This effect is also harnessed to allow cells to be returned to a pluripotent state with roc and loc inhibitors. The papers stated that by combining sirt4 and glutamine it can inhibit the synthesis of glutamine from glutamate intracellularly in the TCA cycle to arrest tumor growth. I believe Quest may have preferred AKG to be used for glutamine deficiency. Since the second stage of his protocol was proliferating redifferentiated cells. But glutamine in its basic form combined with sirt4 could be anticancer. I don't know the applicability in real world.

In the same paper, It mentions that tumors can still use serine to replenish the tca cycle by fostering the synthesis of glutamine from akg. But in breast cancer it states that a PHGDH inhibitor can block this transference as well. A natural PHGDH inhibitor is Withaferin A (aswagandha, ksm-66?) That was the one of a few natural based inhibitors that jumped out at me since it is pretty common.

I remember serine can lower homocysteine, which may be beneficial to many, in spite of its pro cancer properties. Perhaps aswagands cpild be paired with it too.

I don't know if it would be this simple but I vaguely remembered Quest stated his protocol could be seen at as be helpful with cancer as I interpreted it. Reservatrol does upregulate sirt4, atleast it was originally thought it can. Im willing to try combining reservstrol and copper with his progenitor cell redifferntiating protocol. I got good results I felt, till I stupidly restocked my pure resvatrol with a formula with GSE. I was pretty well just confabulating my whole business in my stab at his protocol.

I think Questforlife has taken a lot different avenues in his thread. As he stated he's more interested in pushing the theoretical exploration of telemeres. Being young and healthy he may not need to test out his theories on himself, and understandably doesn't want others to experiment themselves.

I do a lot of wishfully thinking, so I still wonder if some.of hisntheories could be implemented safely.

Edited by bullGenteel, 30 October 2025 - 05:33 AM.