Wow, interesting study I stumbled across. It's from 2004! Can't believe nobody brought it up before.
- Chemically induced long-term potentiation (cLTP) could potentially work by directly stimulating the biochemical machinery that underlies synaptic plasticity, bypassing the need for synaptic activation. Previous reports suggested that agents that raise cAMP concentration might have this capability. We examined the cLTP induced in acute slices by application of Sp-cAMPS or a combination of the adenylyl cyclase activator, forskolin, and the phosphodiesterase inhibitor, rolipram. Under our conditions, cLTP was induced but only if inhibition was reduced. We found that this form of cLTP was blocked by a N-methyl-D-aspartate receptor (NMDAR) antagonist and required the low-frequency test stimulation typically used to monitor the strength of synapses. Interestingly, similar LTP could be induced by lowering the Mg2+ concentration of the ACSF during forskolin/rolipram or Sp-cAMPS application or even by just lowering Mg2+ concentration alone. This LTP was also NMDAR dependent and required only a few (∼5) low-frequency stimuli for its induction. The finding that even low-frequency synaptic stimulation was sufficient for LTP induction indicates that a highly sensitized plasticity state was generated. The fact that some stimulation was required means that potentiation is probably restricted to the stimulated axons, limiting the usefulness of this form of cLTP. However, when similar experiments were conducted using slice cultures, potentiation occurred without test stimuli, probably because the CA3–CA1 connections are extensive and because presynaptic spontaneous activity is sufficient to fulfill the activity requirement. As in acute slices, the potentiation was blocked by an NMDAR antagonist. Our general conclusion is that the induction of LTP caused by elevating cAMP requires presynaptic activity and NMDA channel opening. The method of inducing cLTP in slice cultures will be useful when it is desirable to produce NMDAR-dependent LTP in a large fraction of synapses.
--- Update 3/14/2013 ---
Hello and welcome to the thread that spawned the CILTEP stack. That stands for "Chemically Induced Long TErm Potentiation". This summary is a work in progress.
Here's my current stack:
PDE 4 and others inhibition:
- 2x450 mg Now Artichoke Extract. Other artichoke extract brands have been reported to work too.
- 5mg of Forskolin from 150mg of uncapped Better Body Sports 95% pure C-Bolic capsules. Other forskolin supplements have been reported to work too.
- 1500mg Phenylalanine
- B-Vitamin Complex
- 200mg Caffeine (Optional)
- 350mg Jarrow N-Acetyl L-Tyrosine (Optional)
These are some of the effects myself and others have reported:
- Improved mood and motivation
- Increased ability to study and retain information
- Improved long-term memory
Standard Disclaimer: This is a research thread. The evidence for this stack is largely theoretical and anecdotal. Proceed at your own risk, preferably after reading the whole thread. Please check with your doctor before starting a new supplement regime, especially if you have any health conditions.
Please feel free to add your comments and questions to the thread. The stack is still in active development, as you will see if you visit the later portions of the thread. The thread is a great read and contains lots of interesting scientific and anecdotal information so enjoy it. Thanks!
--- Update 4/09/2013 ---
I have been experimenting with Zembrin as a replacement for Artichoke extract. Zembrin is an extract of the plant Sceletium Tortuosum (a.k.a Kanna). Sceletium Tortuosum grows natively in South Africa and has been used in traditional medicine there for centuries. One of its components, mesembrenone, is a potent and relatively selective PDE4 inhibitor. I have only been using this stack for about a week, but so far results are good. My initial post on the thread about Zembrin is here. I have also made several follow-up posts about my experience. Artichoke Extract is a lot cheaper than Zembrin so it may be a better option for people on a budget. Myself and other users have reported good results with various other Kanna preparations which are also cheaper than Zembrin, so that may be another option. Please note that Zembrin and Kanna affect serotonin via an SSRI like effect and thus should probably not be taken by people who are currently taking SSRIs or other serotonin affecting drugs.
Edited by abelard lindsay, 09 April 2013 - 12:37 PM.